Decreased epidermal AXL expression and increased infiltration of AXL-expressing dendritic cells in psoriasis

IF 1.1 Q4 ALLERGY Journal of Cutaneous Immunology and Allergy Pub Date : 2023-08-25 DOI:10.1002/cia2.12319

Yukiko Ito MD, Sayaka Shibata MD, PhD, Asumi Koyama MD, Lixin Li MD, Eiki Sugimoto MD, Haruka Taira MD, Yuka Mizuno MD, Kentaro Awaji MD, PhD, Shinichi Sato MD, PhD

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Abstract

Background

Psoriasis is a chronic skin inflammatory disease characterized by epidermal proliferation and inflammatory cell infiltration. AXL is a tyrosine kinase receptor that promotes cell proliferation and invasion in cancer cells, and its expression is elevated in multiple tumors. However, less is known about its expression and function in inflammatory diseases.

Objectives

The aim of this study is to examine AXL expression in psoriasis and investigate the biological function of AXL under psoriatic conditions.

Results

AXL mRNA expression was decreased in psoriatic skin compared to healthy skin, and an inverse correlation with neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio was observed. Immunohistochemical staining of psoriatic skin revealed decreased AXL expression of the epidermis and an increased number of AXL-positive dendritic cells in the dermis. Stimulation of epidermal keratinocytes with antimicrobial peptide LL37, but not with IL-17A, resulted in decreased AXL expression. Knockdown of AXL in epidermal keratinocytes did not affect cell proliferation or expression of psoriasis-associated cytokines and chemokines.

Conclusion

Decreased epidermal AXL expression and increased infiltration of AXL-expressing dendritic cells were revealed in psoriasis.

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银屑病患者表皮AXL表达减少，表达AXL的树突状细胞浸润增加

银屑病是一种以表皮增生和炎症细胞浸润为特征的慢性皮肤炎症性疾病。AXL是一种酪氨酸激酶受体，在癌症细胞中促进细胞增殖和侵袭，在多种肿瘤中其表达升高。然而，人们对其在炎症性疾病中的表达和功能知之甚少。本研究的目的是检测AXL在银屑病中的表达，并研究AXL在牛皮癣条件下的生物学功能。与健康皮肤相比，银屑病皮肤中AXL mRNA的表达降低，并且观察到与中性粒细胞与淋巴细胞比率和血小板与淋巴细胞比率呈负相关。银屑病皮肤的免疫组织化学染色显示表皮AXL表达减少，真皮中AXL阳性树突状细胞数量增加。用抗菌肽LL37刺激表皮角质形成细胞，而不是用IL-17A刺激，导致AXL表达降低。表皮角质形成细胞中AXL的敲除不影响细胞增殖或银屑病相关细胞因子和趋化因子的表达。银屑病患者表皮AXL表达降低，表达AXL的树突状细胞浸润增加。

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