Increase in the Level of Orexin Receptor 1 (OX1R) mRNA in the Brain Structures of Rats Prone to Impulsivity in Behavior

IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2022-02-14 DOI:10.1134/S1990750822010085

E. A. Sekste, A. A. Lebedev, E. R. Bychkov, M. I. Airapetov, K. E. Gramota, I. Yu. Tissen, P. D. Shabanov

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引用次数: 1

Abstract—

Orexin and its receptors (OXR) are involved in the mechanisms of pathological craving for alcohol and psychoactive drugs. The orexin system is also involved in the mechanisms of non-chemical forms of addiction: binge eating and gambling. The aim of this study was to investigate the level of orexin receptor mRNA in the hypothalamus, hippocampus, and prefrontal cortex of rats prone to impulsivity in behavior in a model for studying the elements of gambling addiction (a variant of the Iowa Gambling Task test). Brain structures were isolated on the 22nd day of the experiment. The OX1R gene expression was higher in the hypothalamus by 122% and in the hippocampus—by 149% in rats preferring to receive a high reward, but with a low probability as compared with a group of animals that preferred a low level of reinforcement, but with a 100% probability. On the contrary, no significant changes were observed in the level of OX1R mRNA in the prefrontal cortex. The level of OX2R mRNA insignificantly changed in the hypothalamus, hippocampus, and prefrontal cortex of rats prone to impulsivity in behavior. The data obtained suggest involvement of OX1R in the hypothalamus and hippocampus in the mechanisms mediating impulsive behavior and the choice of the significance of positive reinforcement in terms of its varying strength and probability.

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行为冲动大鼠脑结构中食欲素受体1 (OX1R) mRNA水平升高

食欲素及其受体(OXR)参与了对酒精和精神药物的病理性渴求机制。食欲素系统也与非化学形式的成瘾机制有关:暴饮暴食和赌博。本研究的目的是在研究赌博成瘾因素的模型(爱荷华赌博任务测试的一种变体)中，研究易冲动行为的大鼠下丘脑、海马和前额叶皮层中食欲素受体mRNA的水平。实验第22天分离脑结构。OX1R基因在下丘脑和海马体的表达分别高出122%和149%，前者倾向于获得高奖励，但获得高奖励的概率较低，而另一组偏好低水平强化的动物则有100%的概率获得高奖励。相反，前额叶皮层OX1R mRNA水平未见明显变化。易冲动行为大鼠下丘脑、海马和前额叶皮层的OX2R mRNA水平变化不显著。这些数据表明，下丘脑和海马的OX1R参与了冲动行为的调节机制，以及正强化在其强度和概率变化方面的意义选择。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

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0.00%

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期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.