Validated HPTLC Analysis for Estimation of Quercetin in Seeds of Anethum graveolens

Abstract

A high-performance thin layer chromatography (HPTLC) method for the quantification of quercetin was developed on methanolic extract of dill (Anethum graveolens) seeds and subsequently validated. A suitable mobile phase was used to establish the HPTLC method, ethyl acetate, toluene, methanol, chloroform, and formic acid (2:3:3:2). A densitometric analysis was done at 366 nm of wavelength. Quercetin has an Rf value of 0.55. In the dilution range of 100 to 800 ng per band, quercetin revealed a linear connection with r2= 0.9938 in the calibration’s linear regression analysis. By conducting replication analysis on 2 separate days and one day, accuracy was verified. The standard addition method was used to conduct recovery studies to validate accuracy. The quercetin recovery rate was 98.60% on average. Five replicates of each of the three standards were used to detect the system suitability parameter. With regard to both the peak area and the Rf value, the %RSD was observed to be under 2%. The mobile phase concentration was altered from Toluene: Ethyl acetate: Chloroform: Methanol (2:3:3:2) to (3:2:2:3) with few drops of formic acid. It was found to have a %RSD of peak area below 10%, the robustness of the method was assessed. The developed HPTLC method was discovered to be easy to use, precise, accurate, suitable, and robust for estimating quercetin from dill seed extract.