Biological control of Fusarium oxysporum causing damping-off and Pythium myriotylum causing root and crown rot on cannabis (Cannabis sativa L.) plants

IF 1.6 4区 农林科学 Q3 PLANT SCIENCES Canadian Journal of Plant Pathology Pub Date : 2023-02-01 DOI:10.1080/07060661.2023.2172082
C. Scott, Z. Punja
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引用次数: 1

Abstract

Abstract Damping-off, crown rot, and root rot caused by Fusarium oxysporum and Pythium spp. are important diseases that reduce the growth of cannabis plants and cause mortality. There are currently limited management options available to producers in the absence of registered chemical fungicides. The comparative efficacy of five biological-control agents in reducing disease severity was evaluated in this study. Rhapsody® ASO (Bacillus subtilis), Stargus® (Bacillus amyloliquefaciens), Lalstop® (Gliocladium catenulatum, Prestop®), RootShield® Plus WP (Trichoderma harzianum and Trichoderma virens), and Asperello® (Trichoderma asperellum) were applied to cannabis cuttings in rockwool blocks followed 48 hr later by inoculation with a F. oxysporum spore and mycelial suspension. Disease was rated 7, 14 and 21 days post-inoculation. The most effective treatments were Lalstop, Rootshield, Asperello and Stargus, which provided a significant (P < 0.05) reduction (30 to 56.3%) in mean disease severity 14 days post-inoculation compared to the Fusarium inoculated control. Application of Rootshield, Asperello and Lalstop resulted in endophytic colonization of cannabis cuttings when assessed after 2 and 7 days. For Pythium biocontrol, Rootshield Plus WP, Asperello, and Lalstop were applied as a drench to rooted plants 7 days before inoculation with P. myriotylum. Based on disease severity ratings and plant measurements in two experiments, the most effective treatments were Rootshield and Lalstop. Plants receiving Lalstop or Rootshield had significantly (P < 0.05) higher fresh root weights compared to the pathogen only treatment. These results indicate that several biological control agents can protect cannabis plants from infection by Fusarium and Pythium spp. through pre-emptive colonization.
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尖孢镰刀菌(Fusarium oxysporum)和肉豆蔻腐霉(Pythium myrotylum)对大麻(cannabis sativa L.)根腐病的生物防治
摘要由尖孢镰刀菌(Fusarium oxysporum)和霉霉(Pythium spum)引起的枯枯病、冠腐病和根腐病是影响大麻植株生长和致死的重要病害。在没有注册化学杀菌剂的情况下,目前可供生产者选择的管理办法有限。本研究评估了五种生物防治剂在降低疾病严重程度方面的比较功效。将Rhapsody®ASO(枯草芽孢杆菌)、Stargus®(解淀粉芽孢杆菌)、Lalstop®(catenulatum Gliocladium Prestop®)、RootShield®Plus WP(哈茨木霉和绿木霉)和Asperello®(曲霉木霉)应用于岩棉块中的大麻切块,48小时后接种尖孢霉孢子和菌丝悬浮液。接种后7、14和21 d分别进行疾病分级。接种14 d后,与接种镰刀菌的对照相比,Lalstop、Rootshield、Asperello和Stargus处理的平均疾病严重程度显著降低(P < 0.05)(30% ~ 56.3%)。施用Rootshield、Asperello和Lalstop后2天和7天评估大麻插枝的内生定植。根盾+ WP、Asperello和Lalstop在接种myriotyum前7天作为水剂施用于有根植物上,作为防霉剂。根据两个试验的疾病严重程度评分和植物测量,最有效的处理是Rootshield和Lalstop。Lalstop和Rootshield处理的植株鲜根重显著高于单纯病原菌处理(P < 0.05)。这些结果表明,几种生物防治剂可以通过先发制人定植的方式保护大麻植株免受镰刀菌和皮孢菌的感染。
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来源期刊
CiteScore
4.50
自引率
5.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Canadian Journal of Plant Pathology is an international journal which publishes the results of scientific research and other information relevant to the discipline of plant pathology as review papers, research articles, notes and disease reports. Papers may be submitted in English or French and are subject to peer review. Research articles and notes include original research that contributes to the science of plant pathology or to the practice of plant pathology, including the diagnosis, estimation, prevention, and control of plant diseases. Notes are generally shorter in length and include more concise research results. Disease reports are brief, previously unpublished accounts of diseases occurring on a new host or geographic region. Review papers include mini-reviews, descriptions of emerging technologies, and full reviews on a topic of interest to readers, including symposium papers. These papers will be highlighted in each issue of the journal and require prior discussion with the Editor-in-Chief prior to submission.
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