HPLC-UV profiles of Cynanchum auricutalaum, Cynanchum bungei and Cynanchum wilfordii and relationships of their antioxidant activities

IF 0.4 4区医学 Q4 PHARMACOLOGY & PHARMACY Acta poloniae pharmaceutica Pub Date : 2023-07-28 DOI:10.32383/appdr/163096

Pingsu He, Lingchuan Xu, J. Jin, Junyou Jian, Chunmao Yuan, Wei Gu, X. Hao, Lie-jun Huang

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Abstract

The method for simultaneous determination of the two types of principal components, C21- steroids and acetophenones, in Cynanchum bungei, C. auriculatum and C. wilfordii by high-performance liquid chromatography-ultraviolet (HPLC-UV) was developed for the first time. Under the optimized conditions, good linearities (R2≥0.999) were obtained for all analytes, and relative standard deviations (RSDs) of HPLC-UV method validation ranged from 0.01-1.62%. The results of DPPH (1,1-diphenyl 2-picryl hydrazyl) and ABTS (2,2-azobis-3ethylbenzthiazoline-6-sulfonic acid) free radical scavenging assays indicated that the three species had a significant antioxidant activity, with the half maximal effective concentration (EC50) range of 64.56-593.38 μg/mL. Combined with bivariate analysis, the fingerprint-activity relationship of the offline antioxidant activity of the three species with their fingerprints peak was studied, and the results revealed a dose-effect relationship between acetophenones and antioxidant activity. This study provides a theoretical basis for the correct identification and application of the antioxidant activities of C. auriculatum, C. wilfordii and C. bungei.

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金秋花、金秋花和金秋花的HPLC-UV谱图及其抗氧化活性的关系

本文首次建立了高效液相色谱-紫外(HPLC-UV)同时测定金秋草、金秋草和雷公藤中C21-甾体和苯乙酮两类主成分的方法。在优化条件下，所有分析物均具有良好的线性关系(R2≥0.999)，HPLC-UV方法验证的相对标准偏差(rsd)范围为0.01 ~ 1.62%。DPPH(1,1-二苯基- 2-苦硝基肼基)和ABTS(2,2-偶氮-3乙基苯并噻唑-6-磺酸)自由基清除实验结果表明，3种植物均具有显著的抗氧化活性，半数最大有效浓度(EC50)范围为64.56 ~ 593.38 μg/mL。结合双变量分析，研究了三种植物的离线抗氧化活性与指纹峰的指纹-活性关系，结果表明苯乙酮类与抗氧化活性之间存在剂量效应关系。本研究为正确鉴别和应用木耳、雷公藤和黄芪的抗氧化活性提供了理论依据。

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来源期刊

Acta poloniae pharmaceutica 医学-药学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General. A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.