Chemotaxonomic study of the most abundant Egyptian sea-cucumbers using ultra-performance liquid chromatography (UPLC) coupled to high-resolution mass spectrometry (HRMS)

IF 1.6 3区环境科学与生态学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemoecology Pub Date : 2019-12-23 DOI:10.1007/s00049-019-00296-y

Nahla E. Omran, Hoda K. Salem, Samia H. Eissa, Amal M. Kabbash, Manar A. Kandeil, Mohamed A. Salem

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引用次数: 17

Abstract

Actinopyga mauritiana, Bohadschia marmorata, Holothuria leucospilota, H. edulis, H. atra and H. polii are the abundant sea-cucumbers inhabiting either Red or Mediterranean Sea shore in Egypt. The aim of this study was to elucidate the metabolic content in the tegument of the selected sea-cucumbers using ultra-performance liquid chromatography coupled to high-resolution mass spectrometry (UPLC/HRMS). The identified metabolites included sulfated saponins such as holothurin B1 and 24-dehydroechinoside A and non-sulfated saponins such as bivittoside C and D. Our results showed that B. marmorata is substantially different from all the other species and occupies alone one taxon. Consequently, it can be suggested to reclassify the family Holothuriidae into three subfamilies. The first suggested comprising the genera, Holothuria and Actinopyga, the second includes only genus Bohadschia, whereas the third includes only genus Pearsonothuria. Lipidomic analysis of sea-cucumbers showed high variation in saturated and unsaturated fatty acids (USFAs) between the collected species. H. edulis and B. marmorata showed the highest amount of all identified SFAs. Among the USFAs, palmitoleic, docosatrienoic and linoleic acid were detected in varied amount in most species.

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超高效液相色谱-高分辨率质谱联用技术对最丰富的埃及海参进行化学分类研究

毛里求斯放线菌(Actinopyga mauritiana)、marmorata Bohadschia、Holothuria leucospilota、H. edulis、H. atra和H. polii是生活在埃及红海或地中海沿岸的大量海参。本研究采用超高效液相色谱-高分辨率质谱联用技术(UPLC/HRMS)对所选海参被皮中的代谢成分进行了分析。鉴定出的代谢物包括硫酸化皂苷(holothurin B1和24-脱氢棘皮苷A)和非硫酸化皂苷(bivittoside C和d)。结果表明，黑桫椤与其他所有种属存在本质上的差异，并单独占据一个分类单元。因此，可以建议将Holothuriidae科重新划分为三个亚科。第一个可能包括Holothuria和Actinopyga属，第二个可能只包括Bohadschia属，第三可能只包括Pearsonothuria属。脂质组学分析表明，海参的饱和和不饱和脂肪酸含量在不同种类海参中存在较大差异。所有鉴定出的sfa含量最高的是毛竹和毛竹。其中，棕榈烯酸、二十二碳三烯酸和亚油酸在大多数种中含量不同。

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来源期刊

Chemoecology 环境科学-生化与分子生物学

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： It is the aim of Chemoecology to promote and stimulate basic science in the field of chemical ecology by publishing research papers that integrate evolution and/or ecology and chemistry in an attempt to increase our understanding of the biological significance of natural products. Its scopes cover the evolutionary biology, mechanisms and chemistry of biotic interactions and the evolution and synthesis of the underlying natural products. Manuscripts on the evolution and ecology of trophic relationships, intra- and interspecific communication, competition, and other kinds of chemical communication in all types of organismic interactions will be considered suitable for publication. Ecological studies of trophic interactions will be considered also if they are based on the information of the transmission of natural products (e.g. fatty acids) through the food-chain. Chemoecology further publishes papers that relate to the evolution and ecology of interactions mediated by non-volatile compounds (e.g. adhesive secretions). Mechanistic approaches may include the identification, biosynthesis and metabolism of substances that carry information and the elucidation of receptor- and transduction systems using physiological, biochemical and molecular techniques. Papers describing the structure and functional morphology of organs involved in chemical communication will also be considered.