Microsatellite-Based Analysis of Genetic Diversity and Population Structure of Rainbow Trout (Oncorhynchus mykiss) Cultured in Ukraine

Q4 Agricultural and Biological Sciences Genetics of Aquatic Organisms Pub Date : 2020-12-25 DOI:10.4194/2459-1831-v5_1_04

О. Bielikova, S. Тarasjuk, A. Mruk, O. Zaloilo, A. Didenko

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Abstract

The genetic structure of rainbow trout farmed in Ukraine were characterized based on microsatellite loci. The selected set of SSR-markers (Simple-sequence repeatsmarkers) had a high degree of polymorphism that allowed determining the specificity of each local stock (average PIC value = 0.785 ± 0.034). The microsatellite analysis of rainbow trout from the studied stocks showed a high level of genetic diversity (uHe = 0.825 ± 0.030, PIC = 0.785 ± 0.034, I = 1.836 ± 0.127). The level of allelic diversity of the selected loci was high, where the average number of alleles per locus was 7.833, the effective number of alleles per locus was 5.687. The range of amplicon sizes of the studied loci and private alleles for each local group was determined. The cluster analysis showed the presence of three clusters. The range of genetic variability was presented in the graphical interpretation of the principal coordinates analysis (PCoA). Based on unbiased genetic distances, the Kharkiv and Transcarpathian local stocks were found to be the closest, while the Chernivtsi stock was the most distant from them.

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基于微卫星的乌克兰虹鳟遗传多样性和种群结构分析

利用微卫星基因座对乌克兰养殖虹鳟鱼的遗传结构进行了分析。所选择的ssr标记具有高度多态性，可以确定每个本地种群的特异性(平均PIC值= 0.785±0.034)。微卫星分析表明，该种群虹鳟鱼的遗传多样性较高(uHe = 0.825±0.030,PIC = 0.785±0.034,I = 1.836±0.127)。所选基因座的等位基因多样性水平较高，平均等位基因数为7.833个，有效等位基因数为5.687个。确定了每个局部群体的研究位点和私有等位基因的扩增子大小范围。聚类分析显示存在三个聚类。主坐标分析(PCoA)图形化解释了遗传变异的范围。根据无偏遗传距离，哈尔科夫和外喀尔巴阡当地种群被发现是最近的，而切尔诺夫齐种群离它们最远。

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Genetics of Aquatic Organisms Agricultural and Biological Sciences-Aquatic Science

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