Modulatory Effect of Vitamin C on Hypoxia Induced Breast Cancer Stem Cells.

IF 4.7 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2023-11-01 Epub Date: 2023-02-21 DOI:10.34172/apb.2023.073

Masoumeh Kazemi, Soheila Montazersaheb, Mina Noroozpour, Safar Farajnia, Hojjatollah Nozad Charoudeh

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Abstract

Purpose: Eliminating cancer stem cells (CSCs) is a challenge because of their enhanced resistance to anti-cancer drugs. Vitamin C, which is insufficient in patients with higher stages of cancer, has been gaining attention as a potential treatment for human malignancies. Hence this study aimed to analyze the effect of high-dose vitamin C treatment on the gene expression level of HIF-1α, NF-κB1, BAX, and DNMT1 in the MCF7 cells undergoing hypoxia, as an inducer of CSCs characteristics. As a result, vitamin C could be possibly used as a promising therapeutic adjuvant.

Methods: Here we first analyzed the breast CSC population alteration in MCF7 cells following hypoxia induction. Then, we evaluated the impact of vitamin C treatment on the gene expression level of four stemness-related genes in hypoxic MCF7 cells.

Results: Our results indicate that vitamin C could reduce proliferation and stemness states in CSCs possibly by induction of apoptotic markers such as BAX, along with attenuating stemness markers, including NF-κB1, and DNMT1 gene expressions.

Conclusion: According to our findings, vitamin C administration would become a new approach to avoiding the stimulation of CSCs during cancer therapies.

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维生素C对缺氧诱导乳腺癌干细胞的调节作用

目的:消除癌症干细胞(CSCs)是一个挑战，因为它们对抗癌药物的抵抗力增强。晚期癌症患者缺乏维生素C，但作为人类恶性肿瘤的潜在治疗手段，它一直备受关注。因此，本研究旨在分析高剂量维生素C处理对缺氧MCF7细胞中HIF-1α、NF-κB1、BAX和DNMT1基因表达水平的影响，作为CSCs特征的诱导剂。因此，维生素C有可能作为一种有前景的治疗辅助剂。方法:我们首先分析了缺氧诱导后乳腺癌干细胞MCF7细胞群的变化。然后，我们评估了维生素C处理对缺氧MCF7细胞中四个干细胞相关基因表达水平的影响。结果:我们的研究结果表明，维生素C可能通过诱导BAX等凋亡标记物，以及NF-κB1和DNMT1等干性标记物的表达，降低CSCs的增殖和干性状态。结论:根据我们的研究结果，维生素C政府将成为癌症治疗中避免CSCs刺激的新途径。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567

期刊介绍： ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/Abstracted： Web of Science SCIE Scopus CAS INSPEC Portico

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