The Effects of Aerobic Training with Different Intensity on FoxO1 and STRA13 Genes Expression in Subcutaneous Fat Tissue of Male Wistar Rat

Abstract

Background & objective : FoxO1 and STRA13 proteins play an important role in duplication and cellular metabolism, regulation of cell differentiation, apoptosis and reducing the spread of fat tissue in the body. The aim of this study was to investigate the effect of different intensities of aerobic training on the expression level of FoxO1 and STRA13 genes in subcutaneous adipose tissue of male Wistar rats. Methods: In this experimental study, 32 male Wistar rats with a mean age of 8 weeks and weight of 237±33 gr were selected, and they were randomly divided into 4 equal groups including moderate-intensity aerobic training (MIT), high-intensity aerobic training (HIT), high-intensity interval aerobic training (HIIT) and control group. The training program was implemented 8 weeks and 5 sessions per week for the experimental groups,. Adipose tissue biopsy was performed 48 hours after the last training session to evaluate FoxO1 and STRA13 gene expression using RT-PCR method. Data were analyzed using One-way analysis of variance test using SPSS 24 software at the significance level of p <0.05. Results: The results showed that there was a significant difference in the FoxO1 gene expression level in the subcutaneous tissue of male Wistar rats between HIT and control groups ( p =0.0001). However, no significant difference was observed between experimental groups. In addition, there was a significant difference in STRA13 gene expression level in the subcutaneous tissue between MIT ( p =0.008), HIT ( p =0.0001) and HIIT ( p =0.009) groups and control group. Conclusion: According to the results, aerobic exercise with variety of intensity is effective in controlling the genes expression rate involved in fat metabolism and by reducing the FoxO1 and STRA13 genes expression, they cause the duplication and reduce the expansion of fat tissue.