Genetic characterization and pollen viability of a natural putative hybrid of Begonia boliviensis × B. micranthera (Begoniaceae) from Argentina

IF 1.5 4区生物学 Q3 PLANT SCIENCES Botany Letters Pub Date : 2022-10-30 DOI:10.1080/23818107.2022.2135020

A. R. Andrada, V. A. Páez, G. M. Silenzi Usandivaras, M. Tebbitt, P. Albornoz, G. R. Vellicce

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Abstract

ABSTRACT An orange-flowered natural putative hybrid of Begonia boliviensis × B. micranthera was recently discovered from Calilegua National Park in Jujuy Province, Argentina. Propionic hematoxilyn method was applied to study its chromosomal behavior during meiosis, Müntzing’s stain solution was used to determine pollen grain fertility and ITS2 and rbcL sequences were amplified to help confirm the parentage of the putative hybrid. This plant has a gametophytic chromosome number of n = 13, very regular meiotic behavior and an intermediate percentage of viable pollen compared to other studied natural putative hybrids. The ITS2 sequence data support the parentage of the putative hybrid begonia but sequence comparison shows its ITS2 sequence is more similar to B. micranthera than to B. boliviensis. The molecular marker rbcLa revealed that there are no differences between the sequences for B. boliviensis, B. micranthera and the putative hybrid. Begonia boliviensis × B. micranthera is a diploid plant that, due to its moderately high pollen fertility (43.3%) compared to other natural Begonia hybrids (which are often sterile) and regular meiosis, would likely be able to self-cross or cross with other begonia taxa.

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玻利维亚秋海棠×micranthera（秋海棠科）天然杂交种的遗传特性和花粉活力

摘要：最近在阿根廷胡胡伊省卡利莱瓜国家公园发现了一个橙花的玻利维亚秋海棠×薇甘菊的天然杂交种。用原代苏木精法研究其减数分裂过程中的染色体行为，用Müntzing染色液测定花粉粒的育性，并扩增ITS2和rbcL序列以帮助确认假定杂交种的亲本。与其他研究的天然假定杂交种相比，这种植物的配子体染色体数为n=13，减数分裂行为非常规律，花粉存活率中等。ITS2序列数据支持假定的杂交秋海棠的亲本关系，但序列比较表明，其ITS2序列更类似于薇花B.micranthera而不是玻利维亚B.boliviensis。分子标记rbcLa表明，玻利维亚B.micranthera和推定杂交种的序列之间没有差异。玻利维亚秋海棠×micranthera是一种二倍体植物，与其他天然秋海棠杂交种（通常是不育的）相比，其花粉育性中等高（43.3%），并且减数分裂规律，因此很可能能够与其他秋海棠类群自交或杂交。

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来源期刊

Botany Letters Agricultural and Biological Sciences-Plant Science

CiteScore

3.10

自引率

6.70%

发文量

期刊介绍： Botany Letters is an international scientific journal, published by the French Botanical Society (Société botanique de France) in partnership with Taylor & Francis. Botany Letters replaces Acta Botanica Gallica, which was created in 1993, building on over a century of renowned publications by the Société botanique de France.