Effects of long-chain non-coding HIF2PUT on migration and invasion of osteosarcoma cells

Abstract

Objective To observe the effects of long-chain non-coding RNA (lncRNA) HIF2PUT on the proliferation, migration and invasion of osteosarcoma cells. Methods Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of lncRNA HIF2PUT in 47 cases of osteosarcoma tissues (28 males and 19 females, the oldest was 45 years old, the youngest was 8 years old with an average of 20.24 years old) and 2 osteosarcoma cell lines (MG63 and HOS). After screening for stable transfectants by lentivirus, the methyl thiazol tetrazolium (MTT) assay and Transwell assay were applied to detect the effects of lncRNA HIF2PUT on proliferation, migration and invasion of osteosarcoma cells. According to the cell transfection, the experimental group (overexpressed lncRNA HIF2PUT group), the blank transfection group and the blank control group were respectively set. Data were expressed as mean±standard deviations (SD), and SPSS 21.0 software was used for statistical analysis. Results The expression of lncRNA HIF2PUT in 47 cases of osteosarcoma (39.56±0.48) was significantly lower than that in normal tissues (79.56±1.16, t=3.516, P<0.05). The expression level of lncRNA HIF2PUT in MG63 cells was significantly higher than that in HOS cells. Cell function experiments showed that lncRNA HIF2PUT had a significant effect on the proliferation, migration and invasion of osteosarcoma cells. Proliferation of MG63 and HOS cells was significantly inhibited by lncRNA HIF2PUT, and the proliferation index was significantly lower in lncRNA HIF2PUT group than in blank transfection group and blank control group (t=4.785, P<0.05). For the migration experiment, the migrating number of MG63 cells was (154.36±13.85), (196.32±13.52) and (258.63±12.35), respectively (t=3.968, P<0.01), and that of HOS cells was (131.24±10.58), (247.36±17.52) and (224.52±14.52) respectively (t=3.052, P<0.01). For the invasion experiment, the penetrating number of MG63 cells was (73.50±13.89), (98.63±12.57) and (112.30±15.29), respectively (t=23.178, P<0.01), and that of HOS cells was (70.74±13.49) (98.40±16.46) and 89.74±12.47, respectively (t=14.275, P<0.01). The migration and invasion ability of MG63 and HOS cells in lncRNA HIF2PUT group was lower than that in blank transfection group and blank control group. Conclusion The expression of lncRNA HIF2PUT was significantly low in osteosarcoma tissues and MG63 and HOS cell lines. The expression of lncRNA HIF2PUT significantly inhibited the proliferation, migration and invasion of osteosarcoma cells. Key words: Osteosarcoma; Long-chain non-coding RNA; Migration; Invasion