Use of stable isotopes (δ2H, δ13C and δ15N) to infer the migratory connectivity of Terek Sandpipers (Xenus cinereus) at stopover sites in the East Asian–Australasian Flyway

Abstract

Stable isotopes are well documented as effective intrinsic markers to infer migratory connectivity which provides key information for establishing an effective conservation strategy in migratory birds. However, there are few studies using stable isotopes that have been applied to long-distance migratory shorebirds globally and such studies are especially scarce along the East Asian–Australasian Flyway. We used stable isotope analysis (δ2H, δ13C and δ15N) to infer breeding and wintering areas and examine the differences in those values among populations of Terek Sandpipers (Xenus cinereus) at stopover sites in South Korea. The range of δ2H in feathers sampled from birds caught in the Korean peninsula at spring and autumn migration stopover sites was consistent with them being grown at sites throughout their flyway as confirmed by leg flag resightings of birds on this flyway. The eastern Siberia region from Yakutsk to Norilsk and Chukotka in Russia was inferred as the most probable breeding area of the population. Papua New Guinea in the Melanesia region, Malaysia and Indonesia were identified as the most probable wintering areas. Isotope values of populations at different stopover sites and different seasons were consistent. These results suggest that stable isotopes can be effectively used alongside other existing methods (e.g. ringing, coloured leg flags, light level geolocation, satellite tag telemetry) to infer the migratory connectivity for long-distance migratory shorebird species that occur over many countries and continents.