Evaluation of Antioxidant Activity of Ocimum Sanctum-An In Vitro Study

Abstract

--- Introduction: Antioxidants are substances that can prevent or slow damage to cells caused by free radicals, unstable molecules that the body produces as a reaction to environmental and other pressures. They are sometimes called “free-radical scavengers.” The sources of antioxidants can be natural or artificial. Periodontitis results from the loss of balance between microbial virulence factors and a proportionate host response. Antioxidant is a substance that is present at low concentrations which significantly delays or prevents oxidation of that substrate. Ocimum sanctum which is a plant extract had a medicinal value and it had been used in asian countries to treat various disease. Materials and methods: Ocimum Sanctum commercially available powders are used to analyze antioxidant potential. In which three radical scavenging analyzed oxide Results: The results from obtained from the reagents shows DPPH radical scavenging activity shows tulsi shows higher activity compared with vitamin c, Superoxide anion radical scavenging, Nitric oxide radical scavenging activity shows vitamin c has higher activity compared with tulsi. Conclusion: The above results of the antioxidant activity shows that vitamin c activity is higher in Superoxide anion radical scavenging, Nitric oxide radical scavenging activity of each concentration of tulsi 100 𝜇𝑔/ 𝑚𝑙 ,200 𝜇 g/ml,300 𝜇 g/ml,400 𝜇 g/ml,500 𝜇 g/ml. show that Vitamin C has higher concentration compared with tulsi.The results of the study shows that DPPH radical scavenging show the tulsi has highest antioxidant activity compared with Superoxide anion scavenging and Nitric oxide radical scavenging.