Female bovine donor age influence on quality markers’ expression and PPARs abundance in day 7 blastocysts

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Annals of Animal Science Pub Date : 2023-05-12 DOI:10.2478/aoas-2023-0029
Joanna Jaworska, A. Nowicki, I. Kowalczyk-Zięba, D. Boruszewska, A. Siergiej, Milena Traut, K. Łukaszuk, I. Wocławek-Potocka
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Abstract

Abstract In bovine females, the production of embryos derived from oocytes obtained by ovum pick-up (OPU) is becoming a frequent procedure. Collection of oocytes from pre-pubertal animals enables shortening the genetic distance, especially in high-value animals. Nevertheless, the oocyte and later embryo developmental potential differ between oocytes derived from prepubertal and adult heifers. The aim of this study was to determine the possible underlying causes for differences in quality between embryos obtained from pre-pubertal and pubertal heifers. Cumulus oocyte complexes were collected via OPU. The expression of PLAC8, IFNτ, IGFR1, SOX2, and OCT4 in blastocysts was evaluated. The transcriptome of peroxisome proliferator-activated receptors (PPARs), the major genes involved in the metabolism and development of the embryo, and the embryo quality marker gene expression (PLAC8, IFNτ, IGFR1, SOX2, and OCT4) in the day 7 blastocysts derived from oocytes collected from pre-pubertal or pubertal heifers differed significantly. The expression of genes involved in basic biological processes of early embryonic development differed between embryos derived from oocytes collected from pre-pubertal and pubertal heifers. The obtained data may be used to adjust culture conditions to the biological requirements of the embryos derived from oocytes collected from pre-pubertal heifers, and to predict the possibility of successful development of the embryo.
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母牛供体年龄对第7天囊胚质量标志物表达及PPARs丰度的影响
摘要在牛雌性中,从卵子提取(OPU)获得的卵母细胞中生产胚胎正成为一种常见的程序。从青春期前的动物身上采集卵母细胞可以缩短遗传距离,尤其是在高价值动物身上。然而,来自青春期前和成年小母牛的卵母细胞和后期胚胎发育潜力不同。本研究的目的是确定青春期前和青春期小母牛胚胎质量差异的可能潜在原因。通过OPU收集Cumulus卵母细胞复合体。评估PLAC8、IFNτ、IGFR1、SOX2和OCT4在胚泡中的表达。过氧化物酶体增殖物激活受体(PPARs)的转录组、参与胚胎代谢和发育的主要基因以及来自青春期前或青春期小母牛卵母细胞的第7天胚泡中的胚胎质量标记基因表达(PLAC8、IFNτ、IGFR1、SOX2和OCT4)差异显著。参与早期胚胎发育基本生物学过程的基因的表达在从青春期前和青春期小母牛的卵母细胞中获得的胚胎之间存在差异。所获得的数据可用于调整培养条件以适应从青春期前小母牛采集的卵母细胞中获得的胚胎的生物学要求,并预测胚胎成功发育的可能性。
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来源期刊
Annals of Animal Science
Annals of Animal Science 农林科学-奶制品与动物科学
CiteScore
4.00
自引率
5.30%
发文量
138
审稿时长
6-12 weeks
期刊介绍: Annals of Animal Science accepts original papers and reviews from the different topics of animal science: genetic and farm animal breeding, the biology, physiology and reproduction of animals, animal nutrition and feedstuffs, environment, hygiene and animal production technology, quality of animal origin products, economics and the organization of animal production.
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