Antimicrobial activity by solvents extracted from Ocimum basilicum herb against multidrug resistant gram negative rods.

A. Ikram, Sidrah Saleem, Muhammad Imran, Ayesha Ghazal
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引用次数: 1

Abstract

ABSTRACT: Background: Failure of treatment with antibiotics occurs due to increase in number of Multidrug resistant gram negative bacteria, worldwide. The objective of this study was to find out the antimicrobial activity of crude ethanolic extract and its further three fractions by Ocimum basilicum leaves against multi drug resistant gram negative rods. Material and Methods: This descriptive study was conducted in the Department of Microbiology, University of Health Sciences, Lahore from 1st july 2016 to 30th june 2017. Total 80 multidrug resistant gram negative rods were included in this study. Agar dilution method was performed to determine MIC of crude ethanolic extract and different fractions i-e n-hexane, chloroform and ethyl acetate of Ocimum basilicum leaves against multidrug resistant gram negative rods i-e ESBLs and carbapenemase producers. Muti-inoculater was used for inoculation. Results: The mean MICs of crude ethanolic extract, n-hexane fraction, chloroform fraction, and ethyl acetate fraction of Ocimum basilicum against ESBLs were 100.0±8.00, 168.13±8.00, 176.88±8.00 41.75±8.00 respectively. Similarly, the mean MICs of crude ethanolic extract, n-hexane fraction, chloroform fraction, and ethyl acetate fraction of Ocimum basilicum against carbapenemase producers were 77.50±8.00, 113.75±8.00, 132.50±8.00 and 29.50±8.00 respectively. Conclusions: Ethyl acetate fraction and crude ethanolic extract from leaves of Ocimum basilicum showed good antibacterial effectiveness against ESBLs and carbapenem resistant organisms than other fractions. This finding may also promote the effective use of O. basilicum herb and its components in modern medicine.
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罗勒溶剂对多重耐药革兰氏阴性棒的抑菌活性研究。
摘要:背景:世界范围内耐多药革兰氏阴性菌数量增加,导致抗生素治疗失败。本研究的目的是了解罗勒叶的粗乙醇提取物及其另外三个部分对多重耐药革兰氏阴性杆菌的抗菌活性。材料和方法:本描述性研究于2016年7月1日至2017年6月30日在拉合尔卫生科学大学微生物系进行。本研究共纳入80个多药耐药革兰氏阴性杆菌。采用琼脂稀释法测定罗勒叶粗乙醇提取物和不同组分(正己烷、氯仿和乙酸乙酯)对多药耐药革兰氏阴性杆菌ESBLs和碳青霉烯酶产生菌的MIC。采用羊肉接种器进行接种。结果:罗勒粗乙醇提取物、正己烷组分、三氯甲烷组分和乙酸乙酯组分对ESBLs的平均MIC分别为100.0±8.00、168.13±8.00和176.88±8.00 41.75±8.00。类似地,罗勒的粗乙醇提取物、正己烷组分、氯仿组分和乙酸乙酯组分对碳青霉烯酶产生者的平均MIC分别为77.50±8.00、113.75±8.0、132.50±8.00和29.50±8.0。结论:罗勒叶乙酸乙酯组分和粗乙醇提取物对ESBLs和碳青霉烯类耐药菌具有较好的抗菌效果。这一发现也可能促进罗勒草及其成分在现代医学中的有效应用。
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发文量
35
审稿时长
16 weeks
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