Serological monitoring of brucellosis among wild boars in Ukraine during 2019–2020

H. Aliekseieva, A. Pyskun, I. Piankivska, O. Polishchuk, H. Mietolapova
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Abstract

The key to the successful development of animalhusbandry is the epizootic welfare of the country. Forimplementation the set of preventive measures it is requiresa perfect system for diagnosing infectious diseases, includingbrucellosis. Despite the fact that Ukraine is consideredofficially free from this zoonosis among farm animals, thesituation regarding it in the wild fauna of our country remainsinsufficiently studied.This article presents the results of serological studies of548 sera samples of wild boars from 16 regions of Ukraine,collected during 2019 – 2020. The research was conductedon the basis of the State Scientific and Research Institute ofLaboratory Diagnostics and Veterinary and Sanitary Expertise(SSRILDVSE, Kyiv, Ukraine) by using the complementfixation test (CFT) and enzyme-linked immunosorbent assay(ELISA). In addition, all positive samples were further testedon yersiniosis with differential purposes.The systematization of obtained results show thatantibodies to Brucella spp. in sera samples from wild boarsin Ukraine were detected by CFT in 57 samples (10.4 % ofthe total number), by ELISA – in 83 (15.1 %). In general,antibodies to these pathogens were diagnosed in samplesfrom 13 regions (most often from Zhytomyr, Lviv andChernihiv).Positive samples by CFT and/or ELISA werefurther tested for the presence of antibodies to Yersiniaenterocolitica because, according to the literature andguidelines for diagnostic kits, possibility of crossreactions between brucellosis and yersiniosis. As a resultof differential studies, from the 83 positive on brucellosissamples, 42 also showed antibodies to Y. enterocolitica.Taking into account the cross-reactions between tests forbrucellosis and yersiniosis, antibodies to Brucella spp.during the analyzed period were found in 7.5 % of animals(in 41 of 548 samples).The prospect of this work is to further studies of theepizootic situation regarding brucellosis in the wild faunaof Ukraine. The obtained results indicate the circulationof antibodies to this zoonosis among wild boars in ourcountry. Key words: Brucella spp., Yersinia enterocolitica,zoonosis, serological studies, CFT, ELISA, wild fauna.
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2019-2020年乌克兰野猪布鲁氏菌病血清学监测
畜牧业成功发展的关键是国家的流行病福利。为了实施这套预防措施,需要一个完善的传染病诊断系统,包括布鲁氏菌病。尽管乌克兰被认为在农场动物中没有这种人畜共患疾病,但对我国野生动物中有关这种疾病的报道仍进行了充分的研究。本文介绍了2019年至2020年期间从乌克兰16个地区采集的548份野猪血清样本的血清学研究结果。本研究在乌克兰基辅国家劳动诊断兽医卫生专业科学研究院(SSRILDVSE)的基础上,采用补体固定试验(CFT)和酶联免疫吸附试验(ELISA)进行。此外,所有阳性样本都进行了进一步的yersinosis检测,以达到不同的目的。所得结果的系统化表明,CFT在57个样本(占总数的10.4%)和ELISA在83个样本(15.1%)中检测到乌克兰野猪血清样本中的布鲁氏菌属抗体。一般来说,在来自13个地区的样本中诊断出了针对这些病原体的抗体(最常见的是来自日托米尔、利沃夫和切尔尼戈夫)。通过CFT和/或ELISA检测阳性样本是否存在针对小肠结肠炎耶尔森菌的抗体,因为根据诊断试剂盒的文献和指南,布鲁氏菌病和耶尔森菌病之间可能存在交叉反应。作为差异研究的结果,在83例布鲁氏菌感染阳性病例中,42例也显示出小肠结肠炎杆菌抗体。考虑到布鲁氏菌病和耶尔西尼菌病检测之间的交叉反应,在分析期间,7.5%的动物(548个样本中的41个)中发现了布鲁氏菌属抗体。这项工作的前景是进一步研究乌克兰野生动物布鲁氏菌感染的情况。结果表明,这种人畜共患疾病的抗体在我国野猪中存在循环。关键词:布鲁氏菌属,小肠结肠炎耶尔森菌,人畜共患病,血清学研究,CFT,ELISA,野生动物。
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