C. Otitolaiye, A. Omonkhua, K. Oriakhi, E. Okello, I. Onoagbe, F. Okonofua
{"title":"Phytochemical Analysis and in vitro Antioxidant Potential of Aqueous and Ethanol Extracts of Irvingia gabonensis Stem Bark","authors":"C. Otitolaiye, A. Omonkhua, K. Oriakhi, E. Okello, I. Onoagbe, F. Okonofua","doi":"10.5530/pres.15.2.039","DOIUrl":null,"url":null,"abstract":"Background: Irvingia gabonensis extracts are documented to possess antidiabetic, hypocholesterolemic, and antioxidant activities. The phytochemical components of medicinal plants are responsible for their therapeutic properties, particularly those with antioxidant properties. Objectives: The quantitative phytochemicals and in vitro antioxidant properties of the aqueous and ethanol extracts of I. gabonensis stem bark were assessed in this study. Materials and Methods: Standard techniques were used to measure the concentrations of phenols, flavonoids, tannins, alkaloids, steroids, and saponins in water and alcohol extracts of this plant. Total Antioxidant Power (TAP) and the extract's capacity to scavenge free radicals including superoxide, nitric oxide, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzoth iazoline-6-sulfonic acid) (ABTS + ), β-carotene, and hydrogen peroxide were also evaluated. The extracts' Ferric-Reducing Potential (FRAP) was also assessed. Results: The alcohol extract had significantly ( P < 0.05) higher concentrations of all the phytochemicals measured as well as near significantly ( P <0.066) higher TAP in comparison to the aqueous extracts. In general, the in vitro antioxidant capacity of the ethanol extract surpassed that of the water extract. The ethanol extract had IC 50 values that were comparable to or less than the reference standards for its ability to neutralize nitric oxide, H 2 O 2 , superoxide, and DPPH radicals. Conclusion: The outcomes of the quantitative phytochemical analysis and in vitro antioxidant effects of aqueous and ethanol extracts of I. gabonensis stem bark clearly show their potential as an excellent reservoir of bioactive compounds and scavengers of deleterious oxidants; properties that could be explored therapeutically.","PeriodicalId":19813,"journal":{"name":"Pharmacognosy Research","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacognosy Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/pres.15.2.039","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 1
Abstract
Background: Irvingia gabonensis extracts are documented to possess antidiabetic, hypocholesterolemic, and antioxidant activities. The phytochemical components of medicinal plants are responsible for their therapeutic properties, particularly those with antioxidant properties. Objectives: The quantitative phytochemicals and in vitro antioxidant properties of the aqueous and ethanol extracts of I. gabonensis stem bark were assessed in this study. Materials and Methods: Standard techniques were used to measure the concentrations of phenols, flavonoids, tannins, alkaloids, steroids, and saponins in water and alcohol extracts of this plant. Total Antioxidant Power (TAP) and the extract's capacity to scavenge free radicals including superoxide, nitric oxide, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzoth iazoline-6-sulfonic acid) (ABTS + ), β-carotene, and hydrogen peroxide were also evaluated. The extracts' Ferric-Reducing Potential (FRAP) was also assessed. Results: The alcohol extract had significantly ( P < 0.05) higher concentrations of all the phytochemicals measured as well as near significantly ( P <0.066) higher TAP in comparison to the aqueous extracts. In general, the in vitro antioxidant capacity of the ethanol extract surpassed that of the water extract. The ethanol extract had IC 50 values that were comparable to or less than the reference standards for its ability to neutralize nitric oxide, H 2 O 2 , superoxide, and DPPH radicals. Conclusion: The outcomes of the quantitative phytochemical analysis and in vitro antioxidant effects of aqueous and ethanol extracts of I. gabonensis stem bark clearly show their potential as an excellent reservoir of bioactive compounds and scavengers of deleterious oxidants; properties that could be explored therapeutically.
期刊介绍:
Pharmacognosy Research [ISSN: Print -0976-4836, Online - 0974-8490] [http://www.phcogres.com], Quarterly a publication of Phcog.Net is published by Wolters Kluwer - Medknow Publications. It provides peer-reviewed original research articles from the field of Natural Products. The journal serves an international audience of scientists and researchers in a variety of research and academia by quickly disseminating research findings related to Medicinal Plants and Natural Products. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submissions of original contributions in all areas of pharmacognosy are welcome.