Chlorogenic acid improves diabetes-associated muscular atrophy in mice

Abstract

Diabetes is closely relevant to muscle dysfunction. Chlorogenic acid (CGA) possesses various therapeutic functions in diabetic complications. However, no research implied the amelioration of CGA on diabetes-associated muscular atrophy. In this study, diabetic model was established by streptozotocin (STZ) to appraise roles of CGA (30 mg/kg/d for 8 weeks) on diabetes-associated muscular atrophy. Our researches demonstrated that CGA enhanced representative myocyte cross-sections, gastrocnemius weight and grip strength. In serum, CGA restricted CK, LDH, MDA, TNF-a and IL-6 levels, while elevated T-ACO, SOD and CAT levels against diabetic-evoked muscle damage. In skeletal muscle, CGA increased BCL-2 expression, while reduced FBXO-32, MURF-1, DDIT-3, GRP-87 and BAX expressions to improve diabetic muscular atrophy. In conclusion, CGA protected from diabetic-associated muscular atrophy through its bioactivities on the regulation of endoplasmic reticulum stress and apoptosis, and could be a powerful remedy approach in the ameliorations of diabetic muscle atrophy.