Analytical evaluation of Mycobacterium tuberculosis detection in a local comprehensive tuberculosis center following the introduction of genexpert: A cartridge-based nucleic acid amplification test

George Duke Mukoro, WandaliArhyel Dibal
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Abstract

Background: The policies and methods for preliminary evaluation of Mycobacterium tuberculosis (MTB) are evolving as TB becomes resistant to drugs worldwide. Evaluating the effectiveness of New GeneXpert as a first-line protocol for replacing AFB/ZN microscopy in TB evaluation for more system future rollout was paramount for effective TB detection. Aim: The research was a retrospective analytical cohort study, evaluating the detection rates of MTB using Direct-AFB microscopy and GeneXpert among samples from TB suspects. Materials and Methods: Data were collated from the National TB and Leprosy Control Programme register and laboratory records. The study period was from February 2015 to October 2017. The study period was divided into three, 11 months each, representing different preliminary testing policy. Proportions and rates were determined using Microsoft Excel and Chi-square analysis. A P ≤ 0.05 was considered statistically significant. Results: A total of 1931 sample results were analyzed, of which 99.9% were sputum. Nearly 502,578 and 177 samples underwent AFB/ZN microscopy technique, whereas 0, 40, and 634 samples underwent GeneXpert for the respective three cohort periods. The results showed that MTB was present at rates of 8.17%, 5%, and 3.39% for ZN, whereas GeneXpert was 15% and 12.6% for the 2nd and 3rd cohort periods only. In addition, 10% detected by GeneXpert were rifampicin drug resistant, and 50% (4) were placed on therapy for resistant strain GeneXpert improved TB detection significantly. Conclusion: GeneXpert could improve the detection of MTB/RIF strains in developing countries through partnership and global funding for TB/ART centers.
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genexpert试剂盒核酸扩增试验对当地综合性结核病中心结核分枝杆菌检测的分析评价
背景:随着结核病在全球范围内对药物产生耐药性,结核病初步评估的政策和方法正在演变。评估New GeneXpert作为在结核病评估中取代AFB/ZN显微镜的一线方案的有效性,以便在未来推出更多系统,对于有效的结核病检测至关重要。目的:本研究是一项回顾性分析队列研究,使用直接AFB显微镜和GeneXpert评估结核病嫌疑人样本中MTB的检测率。材料和方法:数据来自国家结核病和麻风病控制计划登记册和实验室记录。研究期间为2015年2月至2017年10月。研究期间分为三个月,每个月11个月,代表不同的初步测试政策。使用Microsoft Excel和卡方分析确定比例和比率。P≤0.05被认为具有统计学意义。结果:共分析了1931份样本结果,其中99.9%为痰。近502578和177个样本接受了AFB/ZN显微镜技术,而0、40和634个样本分别在三个队列期接受了GeneXpert。结果显示,ZN的MTB发生率分别为8.17%、5%和3.39%,而GeneXpert仅在第二和第三队列期间分别为15%和12.6%。此外,GeneXpert检测到的10%对利福平具有耐药性,50%(4)对耐药菌株进行治疗GeneXbert显著提高了结核病检测。结论:GeneXpert可以通过合作和全球资助结核病/抗逆转录病毒治疗中心,提高发展中国家MTB/RIF菌株的检测水平。
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来源期刊
自引率
0.00%
发文量
65
审稿时长
20 weeks
期刊介绍: The Nigerian Journal of Medicine publishes articles on socio-economic, political and legal matters related to medical practice; conference and workshop reports and medical news.
期刊最新文献
Knowledge of common cardiovascular diseases and its risk factors among members of a community in South-South Nigeria Prevalence, associated factors, and outcomes of singleton preterm births at a Tertiary Hospital in Port-Harcourt, Nigeria Assessment of factors affecting treatment adherence in patients with chronic kidney disease in Nigeria: A survey from a tertiary health-care centre Oral Neurofibroma: 10-Year Experience in Enugu A community survey of the vaccination status of under-five children in a community in Southern Nigeria
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