Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY Acta Chimica Slovaca Pub Date : 2021-01-01 DOI:10.2478/acs-2021-0008
Laura Jánovová, K. Hroboňová
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Abstract

Abstract Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (RS = 1.9 ) and ristocetin (RS = 1.7 ) in reversed-phase separation mode at the column temperature of 25 °C.
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固定相类型和温度对乳酸对映体HPLC分离的影响
摘要乳酸是一种重要的生物有机酸,以两种对映体形式存在,在人体内代谢不同。本文采用高效液相色谱法直接分离乳酸。采用五种基于大环抗生素的手性固定相进行对映分离,并在5~45°C的不同柱温下测定了保留因子、分辨率和选择性因子等色谱参数。在25°C的柱温下,使用基于替考拉宁(RS=1.9)和利托西汀(RS=1.7)的手性固定相以反相分离模式有效分离乳酸的光学异构体。
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来源期刊
Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
自引率
12.50%
发文量
11
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