Radiosensitization of [Ru(bpy)2(phen)]Cl2 on nasopharyngeal carcinoma cells

Q4 Health Professions Iranian Journal of Radiation Research Pub Date : 2020-07-10 DOI:10.18869/ACADPUB.IJRR.18.3.549

J. Chen, Yang Xiao, L. Wu, Feng-Shou Xiao, Q. Wang, J. Hong, P. Zhu

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Abstract

Background: To investigate effect of radiosensitization of [Ru(bpy)2(phen)] Cl2 complex on nasopharyngeal carcinoma cell line CNE1 and its mechanism. Materials and Methods: Nasopharyngeal carcinoma cell line CNE1 in vitro culture was divided into control group, light irradiation group (4 Gy, 6 MV photonic line), simple metal ruthenium complex treatment group (Ru group, 100 μmol/L [Ru(bpy)2(phen)]Cl2) and metal ruthenium complex combined with radiotherapy group (Combined radiotherapy group, cells were irradiated with 4 Gy and 6 MV photons at 2 days after 100 μmol/L [Ru(bpy)2(phen)]Cl2). Results: Transcriptional level of P53 gene in combined radiotherapy group was higher than that in the other groups (P<0.001). Inhibition rate of combined radiotherapy group was higher than that of Ru group and irradiation group (P<0.001). Apoptotic rate was the highest (P<0.05) in the combined radiotherapy group, and irradiation group was higher than Ru group and control group (P<0.05). Survival rate of Ru group was lower than that of control group under the same radiation dose (P<0.05), and the radiotherapy sensitization ratio was 1.227 (Dq ratio). Conclusion: [Ru(bpy)2 (phen)]Cl2 increases the sensitivity of nasopharyngeal carcinoma cell line CNE1 to X-ray, which may be related to increase of P53 gene expression.

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[Ru（bpy）2（phen）]Cl2对鼻咽癌细胞的放射增敏作用

背景：研究[Ru（bpy）2（phen）]Cl2复合物对鼻咽癌细胞系CNE1的放射增敏作用及其机制。材料与方法：将体外培养的鼻咽癌细胞系CNE1分为对照组、光照组（4Gy，单纯金属钌络合物治疗组（Ru组，100μmol/L[Ru（bpy）2（phen）]Cl2）和金属钌络合物联合放疗组（联合放疗组，在100μmol/L[Ru（bpy。结果：联合放疗组P53基因转录水平高于其他组（P＜0.001），联合放疗组抑瘤率高于Ru组和放疗组（P＞0.001），放疗组高于Ru组和对照组（P<0.05），Ru组在相同剂量下的生存率低于对照组（P<0.01），放疗致敏率为1.227（Dq比）。结论：[Ru（bpy）2（phen）]Cl2可提高鼻咽癌细胞系CNE1对X射线的敏感性，这可能与P53基因表达增加有关。

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来源期刊

Iranian Journal of Radiation Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-

CiteScore

0.67

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Iranian Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.