Genetic diversity of Plum pox virus in Ukraine

Q4 Biochemistry, Genetics and Molecular Biology Biopolymers and Cell Pub Date : 2019-12-31 DOI:10.7124/bc.000a1a

O. Kutsenko, I. Budzanivska, O. Shevchenko

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Abstract

Aim. Establishing the genetic diversity of Plum pox virus (PPV) in Ukraine. Methods. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polymerase chain reaction with reverse transcription (RT-PCR), DNA sequencing and phylogenetic analysis. Results. The samples with visual symptoms of virus infection were collected from different regions of Ukraine and diagnosed using DAS-ELISA. The amplicons of the coat protein (CP) gene were obtained using RT-PCR and sequenced. The obtained viral cDNAs were analyzed using phylogenetic methods to elucidate the genetic diversity of PPV in different regions of Ukraine. The phylogenetic relationships between Ukrainian isolates were studied and compared to PPV isolates from neighboring countries. Conclusions. The PPV strains M and D circulate in Ukraine. For the first time, PPV was found in the Kharkiv region. The PPV isolates circulating in Ukraine are similar to the isolates from Germany, Turkey, Hungary, and Canada. The results can be used to predict the spread of the virus in different Ukrainian regions and in neighboring countries and to establish its origin and predict the development of possible epidemics.

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乌克兰梅花痘病毒的遗传多样性

目标建立乌克兰梅花痘病毒（PPV）的遗传多样性。方法。双抗体夹心酶联免疫吸附试验（DAS-ELISA）、逆转录聚合酶链式反应（RT-PCR）、DNA测序和系统发育分析。后果从乌克兰不同地区采集有病毒感染视觉症状的样本，并使用DAS-ELISA进行诊断。利用RT-PCR方法获得外壳蛋白（CP）基因的扩增子并进行测序。使用系统发育方法对获得的病毒cDNA进行分析，以阐明PPV在乌克兰不同地区的遗传多样性。研究了乌克兰分离株之间的系统发育关系，并与邻国的PPV分离株进行了比较。结论。PPV毒株M和D在乌克兰流行。首次在哈尔科夫地区发现PPV。在乌克兰流行的PPV分离株与来自德国、土耳其、匈牙利和加拿大的分离株相似。该结果可用于预测病毒在乌克兰不同地区和邻国的传播，并确定其起源和预测可能的流行病的发展。

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

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0.00%

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期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.