Insights into the catalytic mechanism of ligninolytic peroxidase and laccase in lignin degradation

Abstract

Abstract The present study aimed to give insights into the binding interactions of the laccases and peroxidase for the degradation of the lignin. The active site of the applied enzymes contains the amino acids that are playing an essential role in lignin degradation. The binding pocket amino acids have interacted with the lignin via the hydrogen, alkyl and van der Waal bonds. The mutagenesis in the active sites of these enzymes predicted the increasing and decreasing performance of the lignin-degrading enzymes. Ramachandran plot analysis of the Laccase and peroxidases determined the active conformation of the lignin-degrading enzymes. Phylogenetic study of the Laccase, lignin peroxidase, versatile peroxidase and manganese peroxidase suggested each of the enzymes belong to the separate protein cluster. The present study reveals the binding potential of the various lignin-degrading enzymes that could increase our understanding of the application of plant biomass large scales.