Protective effect of Allium ochotense Prokh. extract against ethanol-induced cytotoxicity

Q4 Agricultural and Biological Sciences Korean Journal of Food Preservation Pub Date : 2023-06-01 DOI:10.11002/kjfp.2023.30.3.526

Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo

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Abstract

This study aimed to evaluate the antioxidant and hepatoprotective effects of aqueous and 60% ethanol extracts of Allium ochotense Prokh. against alcohol-induced cytotoxicity as well as on the activities of alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl, ferric reducing antioxidant power, and malondialdehyde assays, and found that both extracts exhibited considerable antioxidant activities. Additionally, both extracts showed synergistic effects on the activities of alcohol-metabolic enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde dehydrogenase. In addition, 2’-7’-dichlorodihydrofluorescein diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed that aqueous and 60% ethanol extracts reduced oxidative stress and increased cell viability. Moreover, both extracts regulated the expression of apoptosis-related proteins, namely B-cell lymphoma (BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In conclusion, aqueous and 60% ethanol extracts of A. ochotense Prokh. might be valuable functional materials derived from natural resources for the prevention of ethanol-induced cytotoxicity.

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葱的保护作用。提取物对抗乙醇诱导的细胞毒性

本研究旨在评价大葱水提取物和60%乙醇提取物的抗氧化和保肝作用。对抗酒精诱导的细胞毒性以及酒精代谢酶的活性。用3-乙基苯并噻唑啉-6-磺酸、1,1-二苯基-2-苦基肼、铁还原抗氧化能力和丙二醛测定法分析了提取物的抗氧化作用，发现两种提取物都表现出相当大的抗氧化活性。此外，两种提取物对醇代谢酶（如醇脱氢酶）的活性表现出协同作用，但对醛脱氢酶的活性没有协同作用。此外，2'-7'-二氯二氢荧光素二乙酸酯（DCF-DA）和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）分析显示，水提取物和60%乙醇提取物降低了氧化应激并提高了细胞活力。此外，这两种提取物都调节HepG2细胞中凋亡相关蛋白的表达，即B细胞淋巴瘤（BCl-2）、BCl-2相关X（BAX）和前半胱氨酸蛋白酶-3。总之，A.ochotense Prokh的水提取物和60%乙醇提取物。可能是来源于自然资源的有价值的功能材料，用于预防乙醇诱导的细胞毒性。

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Korean Journal of Food Preservation Agricultural and Biological Sciences-Food Science

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期刊介绍： This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.