{"title":"Isonzyme Changes and DNA Damage Associated with Sterility by Gamma Radiation in Trogoderma granarium Everts Males","authors":"R. Sayed, Nilly A. H. Abdelfattah","doi":"10.3157/021.130.0107","DOIUrl":null,"url":null,"abstract":"ABSTRACT: The present study aimed to investigate the impact of gamma radiation on isoenzymes (peroxidase and polyphenyl oxidase) and DNA damage in Trogoderma granarium. The newly emerged male adults were irradiated with substerilizing and sterilizing doses of gamma radiation for the experiments (100 and 200Gy). The results showed that there was no difference in the distribution of peroxidase and polyphenyl oxidase isoenzymes, and the intensity of peroxidase isoenzymes was the same between the tested samples when compared to the control population and each other. There was a variation in the intensity of the detected polyphenyl oxidase isoenzyme. The DNA electrophoresis showed fragmentation in the gamma irradiated adults as compared to the control. Therefore, it could be concluded that these parameters could be used as a biomarker for identification of gamma irradiated T. granarium males.","PeriodicalId":11719,"journal":{"name":"Entomological News","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomological News","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3157/021.130.0107","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
ABSTRACT: The present study aimed to investigate the impact of gamma radiation on isoenzymes (peroxidase and polyphenyl oxidase) and DNA damage in Trogoderma granarium. The newly emerged male adults were irradiated with substerilizing and sterilizing doses of gamma radiation for the experiments (100 and 200Gy). The results showed that there was no difference in the distribution of peroxidase and polyphenyl oxidase isoenzymes, and the intensity of peroxidase isoenzymes was the same between the tested samples when compared to the control population and each other. There was a variation in the intensity of the detected polyphenyl oxidase isoenzyme. The DNA electrophoresis showed fragmentation in the gamma irradiated adults as compared to the control. Therefore, it could be concluded that these parameters could be used as a biomarker for identification of gamma irradiated T. granarium males.
期刊介绍：
Contributions are accepted on any of the aspects of systematics and taxonomy of insects. This includes: taxonomic revisions, phylogenetics, biogeography and faunistics, and descriptive morphology of insects. Taxonomic papers describing single species are acceptable if such a study is sufficiently justified, however, comprehensive studies that provide a comparative, integrated study of insect systematics and taxonomy will get priority.
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