Role of Polyethylene Glycol and Enzymes in Identifying Antibody Specificity in a Patient with Sickle Cell Disease

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-04-28 DOI:10.1055/s-0043-1772563
Sree Bhagavathi, S. Mukherjee, S. Prakash, A. Sahu, Debasish Mishra, Priyanka Nagrath
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Abstract

Abstract Alloimmunization is a common problem in sickle cell disease (SCD) patients. Providing antigen-negative units in this group of patients makes it challenging for the transfusion laboratory. Antibody identification is required to decrease the incidence of hemolytic transfusion reaction in case of alloimmunization. In SCD patients, identification of antibody specificity is essential to prevent particularly delayed type transfusion reactions. This is a case report of a transfusion-dependent SCD patient whose antibody specificity could not be determined on initial antibody identification testing. A modified antibody identification test was done with polyethylene glycol (PEG) and enzyme to identify antibody specificity in this patient. Alloantibody anti-c was identified and reported to the clinician. The patient was transfused with c antigen-negative blood. This case report highlights the role of PEG and enzyme treatment in antibody identification for transfusion support in this group of highly alloimmunized patients.
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聚乙二醇和酶在镰状细胞病患者抗体特异性鉴定中的作用
摘要异基因免疫是镰状细胞病(SCD)患者的常见问题。在这群患者中提供抗原阴性单位使输血实验室面临挑战。在同种异体免疫的情况下,需要进行抗体鉴定以降低溶血性输血反应的发生率。在SCD患者中,抗体特异性的鉴定对于预防特别延迟型输血反应至关重要。这是一例输血依赖性SCD患者的病例报告,其抗体特异性无法在初始抗体鉴定测试中确定。用聚乙二醇(PEG)和酶进行改良抗体鉴定试验,以鉴定该患者的抗体特异性。鉴定了抗c同种异体抗体,并向临床医生报告。患者输注了c抗原阴性的血液。本病例报告强调了PEG和酶治疗在这组高度同种异体免疫患者的抗体鉴定和输血支持中的作用。
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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0.00%
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99
审稿时长
31 weeks
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