Hypothyroid women have persistently higher oxidative stress compared to healthy controls.

IF 3.5 2区医学 Q2 ENDOCRINOLOGY & METABOLISM European Thyroid Journal Pub Date : 2023-11-03 Print Date: 2023-12-01 DOI:10.1530/ETJ-23-0167

Kamilla R Riis, Camilla B Larsen, Bjarke R Medici, Christian Z Jensen, Kristian H Winther, Emil L Larsen, Christina Ellervik, Jeppe L la Cour, Laszlo Hegedüs, Thomas H Brix, Henrik E Poulsen, Filip K Knop, Birte Nygaard, Steen J Bonnema

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引用次数: 0

Abstract

Objective: Some studies suggest that hypothyroidism is associated with increased oxidative stress. Urinary excretion of 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) represents whole-body RNA and DNA oxidation, respectively. These biomarkers have only been explored sparsely in patients with thyroid disorders.

Methods: In 45 Danish women with newly diagnosed hypothyroidism, we compared 8-oxoGuo and 8-oxodG before or shortly after initiating levothyroxine with the excretion rates at euthyroidism. We also compared the excretion of 8-oxoGuo and 8-oxodG in the patients after restored euthyroidism with 18 healthy control subjects.

Results: Compared with baseline, none of the biomarkers changed significantly in the patients after becoming euthyroid. The geometric mean of 8-oxoGuo was 1.63 (95% CI: 1.49-1.78) nmol/mmol creatinine at baseline and 1.67 nmol/mmol at euthyroidism (95% CI: 1.53-1.83) (P = 0.39), while that of 8-oxodG was 1.28 nmol/mmol creatinine at baseline (95% CI: 1.14-1.44) and 1.32 nmol/mmol at euthyroidism (95% CI: 1.18-1.48), respectively (P = 0.47). The relative mean differences were 0.97 (95% CI: 0.91-1.04) for 8-oxoGuo and 0.97 (95% CI: 0.88-1.06) for 8-oxodG. At baseline, multiple linear regression revealed a positive association between free thyroxine and both biomarkers (8-oxoGuo, P < 0.001; 8-oxodG, P = 0.04). Furthermore, 8-oxoGuo was positively associated with age (P = 0.04) and negatively associated with thyrotropin (P = 0.02). In the control group, the geometric mean of 8-oxoGuo was 1.23 nmol/mmol creatinine (95% CI: 1.07-1.42), while that of 8-oxodG was 1.04 nmol/mmol creatinine (95% CI: 0.88-1.23). Thus, compared with control subjects, euthyroid patients showed a significantly higher level of both 8-oxoGuo (P < 0.001) and 8-oxodG (P = 0.03).

Conclusion: In hypothyroid women, no significant effect of levothyroxine treatment on the oxidative stress biomarkers 8-oxoGuo and 8-oxodG could be demonstrated. However, the excretion of these biomarkers was significantly higher than in healthy controls.

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与健康对照组相比，甲状腺功能减退的女性具有持续更高的氧化应激。

目的：一些研究表明甲状腺功能减退与氧化应激增加有关。尿中8-氧代-7,8-二氢鸟苷（8-oxoGuo）和8-氧代-7，8-二氢-2'-脱氧鸟苷（8-氧代dG）的排泄分别代表全身RNA和DNA氧化。这些生物标志物在甲状腺疾病患者中的研究很少。方法：在45名新诊断为甲状腺功能减退症的丹麦妇女中，我们比较了在开始服用左甲状腺素之前或之后不久的8-氧代郭和8-氧代脱氧葡萄糖与甲状腺功能正常时的排泄率。此外，我们还比较了甲状腺功能正常恢复后患者和18名健康对照受试者的8-氧代郭和8-氧代脱氧葡萄糖的排泄情况。结果：与基线相比，在甲状腺功能正常的患者中，没有任何生物标志物发生显著变化。8-氧代郭的几何平均值在基线时为1.63（95%CI:1.49-1.78）nmol/mmol肌酸酐，在甲状腺功能正常时为1.67 nmol/mol（95%CI:1.53-1.83）（p=0.39），而8-氧代脱氧葡萄糖分别为1.28 nmol/mol肌酸酐（95%CI:1.14-1.44）和1.32 nmol/mmol（95%CI:1.18-1.48。基线时，多元线性回归显示游离甲状腺素与这两种生物标志物呈正相关（8-oxoGuo，P结论：在甲状腺功能减退妇女中，左甲状腺素治疗对氧化应激生物标志物8-oxo郭和8-oxodaG没有显著影响。然而，这些生物标志物的排泄量明显高于健康对照组。

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来源期刊

European Thyroid Journal Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

6.70

自引率

2.10%

发文量

156

期刊介绍： The ''European Thyroid Journal'' publishes papers reporting original research in basic, translational and clinical thyroidology. Original contributions cover all aspects of the field, from molecular and cellular biology to immunology and biochemistry, from physiology to pathology, and from pediatric to adult thyroid diseases with a special focus on thyroid cancer. Readers also benefit from reviews by noted experts, which highlight especially active areas of current research. The journal will further publish formal guidelines in the field, produced and endorsed by the European Thyroid Association.