Biological effect of laser-assisted scar healing (LASH) on standardized human three-dimensional wound healing skin models using fractional non-ablative 1540 nm Er:Glass or 1550 nm diode lasers

IF 2.2 3区医学 Q2 DERMATOLOGY Lasers in Surgery and Medicine Pub Date : 2023-10-19 DOI:10.1002/lsm.23731

Helena I. Riedemann MD, Yvonne Marquardt, Manuela Jansen, Jens M. Baron MD, Sebastian Huth PhD

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Abstract

Purpose

In postoperative wound healing after surgical operations or ablative laser treatments, recent studies suggest the timely use of non-ablative fractional laser treatments with the aim to improve wound healing and prevent pathological scar formation. However, the underlying molecular mechanisms are poorly understood. The aim of this study was to investigate the effects of laser-assisted scar healing (LASH) at the molecular level and to combine it with already established wound healing-promoting local treatments.

Methods

We irradiated full-thickness 3D skin models with a fractional ablative Er:YAG laser to set standardized lesions to the epidermal and upper dermal layer. Subsequently, LASH was induced by irradiating the models with either a fractional non-ablative 1540 nm Er:Glass or 1550 nm diode laser. In addition, we tested the combination of non-ablative fractional laser treatment and topical aftercare with a dexpanthenol-containing ointment (DCO).

Results

Histological analysis revealed that models irradiated with the 1540 nm Er:Glass or 1550 nm diode laser exhibited accelerated but not complete wound closure after 16 h. In contrast, additional topical posttreatment with DCO resulted in complete wound closure. At gene expression level, both non-ablative laser systems showed similar effects on epidermal differentiation and mild anti-inflammatory properties. The additional posttreatment with DCO enhanced the wound-healing effects of LASH, especially the upregulation of epidermal differentiation markers and anti-inflammatory cytokines at the gene expression level.

Conclusion

This in vitro study deciphers the biological effects of LASH with a fractional non-ablative 1540 nm Er:Glass or a 1550 nm diode laser in 3D skin models. These data help to better understand the biological properties of the LASH technique and is important to optimize its application.

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激光辅助瘢痕愈合（LASH）对标准化人体三维创伤愈合皮肤模型的生物学效应 nm Er：玻璃或1550 nm二极管激光器。

目的：在外科手术或消融激光治疗后的术后伤口愈合中，最近的研究建议及时使用非消融部分激光治疗，以改善伤口愈合并防止病理性瘢痕形成。然而，人们对其潜在的分子机制知之甚少。本研究的目的是在分子水平上研究激光辅助瘢痕愈合（LASH）的效果，并将其与已经建立的促进伤口愈合的局部治疗相结合。方法：我们用部分消融Er:YAG激光照射全厚度3D皮肤模型，在表皮和上真皮层设置标准化病变。随后，通过用部分非消融1540照射模型来诱导LASH nm Er：玻璃或1550 nm二极管激光器。此外，我们测试了非消融部分激光治疗和含右泛醇软膏（DCO）的局部善后处理的组合。结果：组织学分析显示，1540 nm Er：玻璃或1550 nm二极管激光器在16岁后表现出加速但未完全闭合伤口 h.相反，DCO的额外局部后处理导致伤口完全闭合。在基因表达水平上，两种非消融激光系统对表皮分化和轻度抗炎特性表现出相似的效果。DCO的额外后处理增强了LASH的伤口愈合效果，特别是在基因表达水平上调表皮分化标志物和抗炎细胞因子。结论：这项体外研究用部分非消融1540破解了LASH的生物学效应 nm Er：玻璃或1550 纳米二极管激光在3D皮肤模型中的应用。这些数据有助于更好地了解LASH技术的生物学特性，对优化其应用非常重要。

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来源期刊

Lasers in Surgery and Medicine 医学-外科

CiteScore

5.40

自引率

12.50%

发文量

119

审稿时长

1 months

期刊介绍： Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.