Genetic Profile of FOXO3 Single-Nucleotide Polymorphism in Colorectal Cancer Patients.

IF 2.5 3区医学 Q3 ONCOLOGY Oncology Pub Date : 2024-01-01 Epub Date: 2023-10-19 DOI:10.1159/000533729

Laraib Uroog, Arshad Husain Rahmani, Mohammed A Alsahli, Saleh A Almatroodi, Rauf Ahmad Wani, M Moshahid Alam Rizvi

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Abstract

Introduction: Colorectal cancer (CRC) heritability is determined by the composite relations between inherited variants and environmental factors. In developing countries like India, the incidence rates of CRC are especially increasing. In this study, we have focused on the distribution of the FOXO3 gene polymorphisms among the patients with CRC in North India.

Methods: A case-control study was conducted on 487 CRC patients and 487 age-matched controls. We genotyped single-nucleotide polymorphisms rs2253310 and rs4946936 through polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis and PCR-single-stranded conformation polymorphism procedure followed by sequence detection.

Results: A significantly increased risk of CRC was observed for the CC genotype of the rs4946936 polymorphism compared to the TT genotype (p = 0.02; odd ratio [OR] = 1.40, confidence interval [CI] = 1.05-1.87). GT haplotype appeared to be a "risk" haplotype (OR = 1.71, 95% CI = 0.82-2.19), while as other haplotypes CC (OR = 0.83, 95% CI = 0.32-1.54), CT (OR = 0.75, 95% CI = 0.25-1.01), and GC (OR = 0.98, 95% CI = 0.88-1.14) were found to be "protective" for developing CRC.

Conclusion: This study suggests an association of increased risk of CRC with the rs4946936 polymorphism but not with the rs2253310 polymorphism.

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结直肠癌癌症患者FOXO3单核苷酸多态性的遗传图谱。

引言：癌症遗传力是由遗传变异与环境因素之间的复合关系决定的。在像印度这样的发展中国家，CRC的发病率特别高。在本研究中，我们重点研究了FOXO3基因多态性在北印度癌症结直肠癌患者中的分布。方法：对487例癌症结直肠癌患者和487例年龄匹配的对照进行病例对照研究。我们通过聚合酶链式反应限制性片段长度多态性（RFLP）分析和聚合酶链式反应单链构象多态性（SSCP）程序以及序列检测对SNPs rs2253310和rs4946936进行基因分型。结果：与TT基因型相比，rs4946936多态性的CC基因型患CRC的风险显著增加（p=0.02；OR=1.40 CI=1.05-1.87）。GT单倍型是一种“危险”单倍型（OR-1.71，95%CI=0.82~2.19），而其他单倍型CC（OR-0.83，95%CI=0.32~1.54]），CT（OR-0.75，95%CI=0.25～1.01）和GC（OR-0.98，95%CI=0.88～1.14）对癌症的发生具有保护作用。结论：本研究表明rs4946936多态性与CRC风险增加有关，而rs2253310多态性和CRC风险增加无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oncology 医学-肿瘤学

CiteScore

6.00

自引率

2.90%

发文量

审稿时长

6-12 weeks

期刊介绍： Although laboratory and clinical cancer research need to be closely linked, observations at the basic level often remain removed from medical applications. This journal works to accelerate the translation of experimental results into the clinic, and back again into the laboratory for further investigation. The fundamental purpose of this effort is to advance clinically-relevant knowledge of cancer, and improve the outcome of prevention, diagnosis and treatment of malignant disease. The journal publishes significant clinical studies from cancer programs around the world, along with important translational laboratory findings, mini-reviews (invited and submitted) and in-depth discussions of evolving and controversial topics in the oncology arena. A unique feature of the journal is a new section which focuses on rapid peer-review and subsequent publication of short reports of phase 1 and phase 2 clinical cancer trials, with a goal of insuring that high-quality clinical cancer research quickly enters the public domain, regardless of the trial’s ultimate conclusions regarding efficacy or toxicity.