An efficient dual-function fluorescent probe for sulfites and sulfides and its imaging application in cells.

IF 4.6 Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2024-01-15 Epub Date: 2023-10-16 DOI:10.1016/j.saa.2023.123541

Bing-Yu Wei, Cong-Yao Zhao, Meng-Min Xiao, Yi Zheng, Feng Li, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin

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引用次数: 1

Abstract

As gas signaling molecules in organisms, SO₂ derivatives and H₂S play crucial regulating roles in a series of physiological processes. Therefore, developing an assay that can accurately monitor the concentration of SO₂ derivatives and H₂S in cells is extremely important for the research and treatment of related illnesses. A bifunctional probe SN-F based on FRET mechanism for SO₂ derivatives and H₂S was designed. SN-F had a short response time to SO₂ (2 min), excellent anti-interference capability and selectivity in the non-organic solvent system (pH = 7.4), which was suitable for the determination of SO₂ derivatives in cells. SN-F had a wide linear range for H₂S. Moreover, SN-F was applied in cell imaging successfully with high targeting ability to endoplasmic reticulum (ER) and could monitor endogenous and exogenous H₂S in cells.

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一种高效的亚硫酸盐和硫化物双功能荧光探针及其在细胞成像中的应用。

SO2衍生物和H2S作为生物体内的气体信号分子，在一系列生理过程中起着至关重要的调节作用。因此，开发一种能够准确监测细胞中SO2衍生物和H2S浓度的检测方法，对于相关疾病的研究和治疗至关重要。设计了一种基于FRET机理的SO2衍生物和H2S双功能探针SN-F。SN-F对SO2的响应时间短（2min），在非有机溶剂体系（pH=7.4）中具有良好的抗干扰能力和选择性，适用于细胞中SO2衍生物的测定。SN-F对H2S具有较宽的线性范围。此外，SN-F以其对内质网（ER）的高靶向能力成功应用于细胞成像，并可监测细胞内内源性和外源性H2S。

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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

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