Hsa_circ_0007823 Overexpression Suppresses the Progression of Triple-Negative Breast Cancer via Regulating miR-182-5p-FOXO1 Axis.

IF 3.3 4区 医学 Q2 ONCOLOGY Breast Cancer : Targets and Therapy Pub Date : 2023-10-18 eCollection Date: 2023-01-01 DOI:10.2147/BCTT.S417547
Jinling Yu, Haofeng Wang, Weida Shen, Yingzi Zhou, Jing Cui, Haichuan Li, Beimin Gao
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Abstract

Background: This study aimed to analyze the specific expression of hsa_circ_0007823 in triple-negative breast cancer (TNBC) and explore the roles and related molecular mechanisms of hsa_circ_0007823 in TNBC.

Materials and methods: Relative hsa_circ_0007823 levels in TNBC tissues and cell lines were examined by reverse transcription-quantitative polymerase chain reaction. The value of hsa_circ_0007823 levels was evaluated in patients' clinicopathological characteristics and prognostic prediction. A dual-luciferase reporter assay was used to determine the relationship between hsa_circ_0007823, miR-182-5p, and FOXO1. The effect of circ_0007823 overexpression on the growth of TNBC cells was investigated in vitro and in vivo.

Results: Lower levels of hsa_circ_0007823 were found in TNBC tissues and cell lines and were closely associated with lymph node metastasis, poorer overall and disease-free survival rates. MiR-182-5p was significantly up-regulated, whereas FOXO1 was down-regulated in TNBC cell lines. The miR-182-5p inhibition up-regulated FOXO1 in TNBC cells. Dual-luciferase reporter assays showed that hsa_circ_0007823, miR-182-5p, and FOXO1 interacted with each other. Overexpression of circ_0007823 significantly inhibited the viability, migration, and invasion of TNBC cell lines, but promoted apoptosis. In vivo experiments showed that circ_0007823 overexpression inhibited tumor growth and down-regulated miR-182-5p and up-regulated FOXO1.

Conclusion: Hsa_circ_0007823 overexpression could suppress the growth, invasion, and migration of TNBC cells, and inhibit tumor growth by regulating miR-182-5p/FOXO1.

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Hsa_cir_0007823过表达通过调节miR-182-5p-FOXO1轴抑制三阴性乳腺癌症的进展。
背景:本研究旨在分析hsa_cir_0007823在癌症三阴性组织中的特异性表达,探讨hsa_ccirc_0007823在TNBC中的作用及其相关分子机制。评估hsa_cir_0007823水平在患者临床病理特征和预后预测中的价值。双荧光素酶报告基因测定用于确定hsa_cir_0007823、miR-182-5p和FOXO1之间的关系。在体外和体内研究了circ_0007823过表达对TNBC细胞生长的影响。结果:在TNBC组织和细胞系中发现hsa_cir_0007823水平较低,与淋巴结转移、较差的总体生存率和无病生存率密切相关。在TNBC细胞系中,MiR-182-5p显著上调,而FOXO1下调。miR-182-5p的抑制上调了TNBC细胞中的FOXO1。双荧光素酶报告基因分析显示,hsa_cir_0007823、miR-182-5p和FOXO1相互作用。circ_0007823的过表达显著抑制了TNBC细胞系的活力、迁移和侵袭,但促进了细胞凋亡。体内实验表明,circ_0007823过表达抑制肿瘤生长,下调miR-182-5p,上调FOXO1。
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来源期刊
CiteScore
4.10
自引率
0.00%
发文量
40
审稿时长
16 weeks
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