Formulation and Characterization of a Novel Oxidized Alginate-Gelatin-Silk Fibroin Bioink with the Aim of Skin Regeneration.

Q2 Biochemistry, Genetics and Molecular Biology Iranian Biomedical Journal Pub Date : 2023-09-01 Epub Date: 2023-08-23 DOI:10.61186/ibj.27.5.280

Khadijeh Sanaei, Ali Zamanian, Shohreh Mashayekhan, Tayebe Ramezani

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Abstract

Background: In the present study, a novel bioink was suggested based on the oxidized alginate (OAlg), gelatin (GL), and silk fibroin (SF) hydrogels.

Methods: The composition of the bioink was optimized by the rheological and printability measurements, and the extrusion-based 3D bioprinting process was performed by applying the optimum OAlg-based bioink.

Results: The results demonstrated that the viscosity of bioink was continuously decreased by increasing the SF/GL ratio, and the bioink displayed a maximum achievable printability (92 ± 2%) at 2% (w/v) of SF and 4% (w/v) of GL. Moreover, the cellular behavior of the scaffolds investigated by MTT assay and live/dead staining confirmed the biocompatibility of the prepared bioink.

Conclusion: The bioprinted OAlg-GL-SF scaffold could have the potential for using in skin tissue engineering applications, which needs further exploration.

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以皮肤再生为目的的新型氧化海藻酸明胶丝素生物墨水的配方和表征。

背景：在本研究中，提出了一种基于OAlg、GL和SF水凝胶的新型生物墨水。方法：通过流变学和可打印性测量对生物墨水的组成进行优化，并应用优化的OAlg基生物墨水进行基于挤出的3D生物打印工艺。结果：实验结果表明，随着SF/GL比例的增加，生物墨水的粘度不断降低，在2%（w/v）的SF和4%（w/v）的GL下，生物墨水显示出最大可打印性（92±2%）。此外，MTT法和活/死染色对支架的细胞行为进行了研究，证实了所制备的生物墨水的生物相容性。结论：生物打印OAlg-GL-SF支架在皮肤组织工程中具有应用潜力，有待进一步探索。

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