Histological artifacts associated with laser and electroscalpel gingivectomy: Case series

Jennifer Orozco, David Rico, Lía Barrios, Vivi Hoyos, Pilar Blanco
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Abstract

Introduction. Over time, efforts have been invested in the design of new instruments that overcome the disadvantages of the gold standard instrument in surgery, the scalpel. As a result, electronic equipment has emerged such as the electric scalpel and laser devices. The available evidence on these instruments suggests that the tissue response is related to each instrument’s physical and biological cutting principles. Objective. To compare the histological changes in gingiva samples associated with surgical cutting performed with a 940 nm diode laser, a 2780 nm erbium, chromium: yttriumscandium-gallium-garnet (Er,Cr:YSGG) laser, and an electric scalpel, by presenting a series of cases. Case presentation. We present three cases of healthy patients undergoing cosmetic surgery. The clinical examination revealed exposure of a keratinized gingiva band greater than 4 mm, normal color and texture in gingival tissue, with a firm consistency and no bleeding on periodontal probing. Gingivectomy was indicated with the following protocols: Diode laser of 940 nm at 1 W, in continuous mode; Er,Cr:YSGG laser of 2780 nm at 2.5 W, 75 Hz, H mode, air 20, water 40, gold tip MT4); and electric scalpel in cutting mode at power level four. Gingival tissue samples were taken and stored in 10% formaldehyde for histological analysis. Conclusion. All the evaluated cutting instruments generated histological changes produced by the thermal effect, the main ones being collagen coagulation and carbonization. The depth of thermal damage caused by the 2780 nm Er,Cr:YSGG laser was much lesser than that induced by the electric scalpel and the 940 nm diode laser.

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激光和电刀牙龈切除术相关的组织学伪影:一系列病例。
介绍。历史上,人们一直在努力设计新的仪器,以克服外科参考标准手术刀的缺点。因此,出现了电刀和各种激光技术设备等电子设备。关于这些仪器的现有信息表明,受干预组织的反应受到仪器切割的物理和生物学原理的影响。目标。通过一系列病例的介绍,比较与940 nm二极管激光、铒激光、铬:钇-钪-石榴石(Er,Cr:YSGG)(2780nm)和电刀进行手术切割相关的牙龈样本的组织学变化。病例介绍。报告了3例接受美容手术的健康患者。临床检查显示,在牙周穿刺时暴露了一条大于4毫米的角化牙龈带,牙龈组织颜色和质地正常,稠度稳定,无出血。牙龈切除术采用以下方案:二极管激光940 nm至1 W,连续模式;Er激光器,Cr:YSGG,2780 nm至2.5 W,75 Hz,H模式,空气20,水40,金尖MT4;和切割模式的电刀,四级。采集牙龈组织样本,储存在10%甲醛中进行组织学分析。结论。这三种切割工具都产生了热效应引起的组织学变化;主要是胶原凝固和炭化。
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