A. Rangel , M. Lam , A. Hocini , V. Humblot , K. Ameyama , V. Migonney , G. Dirras , C. Falentin-Daudre
{"title":"Bioactivation of New Harmonic Titanium Alloy to Improve and Control Cellular Response and Differentiation","authors":"A. Rangel , M. Lam , A. Hocini , V. Humblot , K. Ameyama , V. Migonney , G. Dirras , C. Falentin-Daudre","doi":"10.1016/j.irbm.2023.100771","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><span>The purpose of this research article is to present the functionalization of a new titanium alloy of the system TiNbZr, by the grafting of a bioactive polymer (poly(sodium styrene sulfonate), PNaSS) using the “grafting from” technique to improve the </span>osseointegration. The resulting grafted polymer is covalently bonded to the substrate in this procedure thanks to surface-induced polymerization.</p></div><div><h3>Material and Method</h3><p>Colorimetric assay<span>, Fourier-transform infrared spectra recorded in attenuated total reflection mode (ATR-FTIR), Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements (WCA) were applied to characterize the surfaces. In addition, the effect of the grafting on the biological response was assessed using MC3T3-E1 pre-osteoblast cells line.</span></p></div><div><h3>Results</h3><p>This study showed that grafting rates obtained on these new alloy are as good (around 4.5 μg/cm<sup>2</sup>) as on classical alloys. In parallel, <em>in vitro</em><span><span> biological response study was carried out to assess toxicity, cell viability<span>, and morphology on titanium alloys TiNbZr functionalized. Moreover, results showed superior alkaline phosphatase activity and higher calcium deposition on grafted samples, implying a beneficial effect of the PNaSS in </span></span>osteoinduction activity.</span></p></div><div><h3>Conclusions</h3><p>Grafted TiNbZr improves the cell response, in particular, the osseointegration.</p></div>","PeriodicalId":14605,"journal":{"name":"Irbm","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Irbm","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1959031823000209","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 1
Abstract
Objective
The purpose of this research article is to present the functionalization of a new titanium alloy of the system TiNbZr, by the grafting of a bioactive polymer (poly(sodium styrene sulfonate), PNaSS) using the “grafting from” technique to improve the osseointegration. The resulting grafted polymer is covalently bonded to the substrate in this procedure thanks to surface-induced polymerization.
Material and Method
Colorimetric assay, Fourier-transform infrared spectra recorded in attenuated total reflection mode (ATR-FTIR), Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements (WCA) were applied to characterize the surfaces. In addition, the effect of the grafting on the biological response was assessed using MC3T3-E1 pre-osteoblast cells line.
Results
This study showed that grafting rates obtained on these new alloy are as good (around 4.5 μg/cm2) as on classical alloys. In parallel, in vitro biological response study was carried out to assess toxicity, cell viability, and morphology on titanium alloys TiNbZr functionalized. Moreover, results showed superior alkaline phosphatase activity and higher calcium deposition on grafted samples, implying a beneficial effect of the PNaSS in osteoinduction activity.
Conclusions
Grafted TiNbZr improves the cell response, in particular, the osseointegration.
期刊介绍：
IRBM is the journal of the AGBM (Alliance for engineering in Biology an Medicine / Alliance pour le génie biologique et médical) and the SFGBM (BioMedical Engineering French Society / Société française de génie biologique médical) and the AFIB (French Association of Biomedical Engineers / Association française des ingénieurs biomédicaux).
As a vehicle of information and knowledge in the field of biomedical technologies, IRBM is devoted to fundamental as well as clinical research. Biomedical engineering and use of new technologies are the cornerstones of IRBM, providing authors and users with the latest information. Its six issues per year propose reviews (state-of-the-art and current knowledge), original articles directed at fundamental research and articles focusing on biomedical engineering. All articles are submitted to peer reviewers acting as guarantors for IRBM''s scientific and medical content. The field covered by IRBM includes all the discipline of Biomedical engineering. Thereby, the type of papers published include those that cover the technological and methodological development in:
-Physiological and Biological Signal processing (EEG, MEG, ECG…)-
Medical Image processing-
Biomechanics-
Biomaterials-
Medical Physics-
Biophysics-
Physiological and Biological Sensors-
Information technologies in healthcare-
Disability research-
Computational physiology-
…
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
