Effects of exogenous oxytocin on the semen characteristics of the Indonesian wild cattle, banteng (Bos javanicus), collected by electroejaculation: Implications for semen collection techniques and genome resource banking

James D. Gillis , William V. Holt , Lisa Yon , Gwen E. Myers , Rodney Schnellbacher , Rhudy Holly , David Love , Linda M. Penfold
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外源催产素对电射精采集印尼野牛巴腾(Bos javanicus)精液特征的影响:对精液采集技术和基因组资源库的影响
制定协议,最大限度地提高濒危物种精液采集过程中的精子总产量,对于战略性基因组资源库实践至关重要。在精液采集之前,已对家养牛种给予外源性催产素,以增加单次采集的精子总数。本研究中验证的假设是,在通过电射精(EEJ)收集精液之前给予外源性催产素将显著增加从班腾获得的精液的总精子数。Banteng在EEJ前~10分钟静脉注射外源性催产素(50 i.u.)。评估了13个班滕(n=6个催产素治疗组,n=8个未治疗组)的精液体积、pH、渗透压、精子浓度、精子总数、活动精子百分比、状态(0-5分;0=无向前进展,5=快速直接进展)。在班腾精液中,催产素处理组在体积、精子浓度和精子总数方面存在显著差异(p<0.05)(平均值±SEM;17.5±3.7 ml,2138±883.9×106精子/ml,32070 ± 8812×106精子)和未经处理的(9.1 ± 1.8 308.8毫升 ± 69.3×106精子/ml,2702 ± 763.7×106精子)公牛。这些发现表明,在精液采集前给班腾注射催产素会显著增加精子总数,如果常规使用,将最大限度地增加基因组资源库中可用的班腾精子数量。在三种催产素治疗的班藤中,在EEJ程序开始前,在包皮上观察到了精液,这表明在其他精液采集技术(如导尿)之前,催产素可能是一种有用的药物。
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Theriogenology wild
Theriogenology wild Animal Science and Zoology, Veterinary Science
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