The use of single-cell sequencing to reveal stem/progenitor cells in animal organ regeneration

Abstract

Advances in single-cell sequencing technologies have made it possible to reveal the cellular basis of complex organ regeneration processes. In this review, we briefly introduce commonly used high-throughput single-cell sequencing platforms and their characteristics. We discuss in depth how single-cell sequencing techniques can be used in the study of regeneration to reveal stem/progenitor cell types and their developmental trajectories. From the perspective of stem cells, we review animal models of regenerative biology and organ models involved in regenerative medicine, with a focus on limb/fin/digit-tip regeneration. Finally, we discuss previous prospective work on improving systematic approaches to explore the origin and evolution of cellular diversity, as well as future research directions.