Antihyperglycemic and anti-type 2 diabetic activity of marine hydroquinone isolated from brown algae (Dictyopteris polypodioides)

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional and Complementary Medicine Pub Date : 2023-07-01 DOI:10.1016/j.jtcme.2023.03.007

Thi Phuong Thao Truong, Thanh Men Tran, Thi Xuan Trang Dai, Chi Linh Tran

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引用次数: 1

Abstract

Background and aims

Brown algae (Dictyopteris polypodioides) extract (DP) presented high inhibitory potential against α-amylase. The present study aims to isolate, purify and evaluate the antihyperglycemic and anti-type 2 diabetic activities of marine hydroquinone from DP.

Methods

Marine hydroquinones were isolated using silica gel, HPLC, and NMR spectroscopy was used to identify compound 1 and compound 2 as zonarol and isozonarol, respectively. The anti-hyperglycemic and anti-type 2 diabetic activities of zonarol were investigated by in vitro assay (α-amylase, α-glucosidase), Lineweaver–Burk plot and Type 2 diabetes mellitus model (T2DM) mice induced by streptozotocin (STZ).

Result

Zonarol had the highest content and the strongest inhibitory activity against α-glucosidase (IC₅₀ value of 6.03 mg L⁻¹) and α-amylase (IC₅₀ value of 19.29 mg L⁻¹) in a competitive inhibition and mix-type manner, respectively. The maltose and starch loading tests revealed that zonarol significantly reduced postprandial glycemia after 30 min loading (9.12 and 8.12 mg/dL, respectively), compared to normal (11.37 and 12.37 mg/dL, respectively). Zonarol exhibited pancreatic islet cell rejuvenation, as evidenced by increased pancreatic islet mass, and hence helps in the restoration of insulin levels and therefore improves the glucose metabolism in STZ-induced diabetic mice. Zonarol treatment in T2DM elevated abundant levels of main SCFAs (propionate, butyrate, and valeric acid), which are closely related to glucose metabolism homeostasis.

Conclusion

Our finding indicates that zonarol could be used as a food supplement to treat hyperglycemia and diabetes.

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褐藻海洋对苯二酚抗高血糖和2型糖尿病活性研究

背景与目的长藻提取物对α-淀粉酶具有较高的抑制作用。本研究旨在从DP.中分离、纯化并评价海洋对苯二酚的抗高血糖和抗2型糖尿病活性。方法用硅胶、高效液相色谱和核磁共振波谱分离海洋对苯二酚，分别鉴定化合物1和化合物2为zonarol和isozonarol。用α-淀粉酶、α-葡萄糖苷酶、α-，Lineweaver–Burk图和链脲佐菌素诱导的2型糖尿病模型（T2DM）小鼠。麦芽糖和淀粉负荷测试显示，与正常情况（分别为11.37和12.37 mg/dL）相比，zonarol在负荷30分钟后显著降低了餐后血糖（分别为9.12和8.12 mg/dL。Zonarol表现出胰岛细胞再生，胰岛质量增加证明了这一点，因此有助于恢复胰岛素水平，从而改善STZ诱导的糖尿病小鼠的葡萄糖代谢。Zonarol治疗T2DM提高了大量的主要SCFA（丙酸、丁酸和戊酸）水平，这些SCFA与葡萄糖代谢稳态密切相关。结论zonarol可作为治疗高血糖和糖尿病的食品补充剂。

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