Preclinical evaluation of Hibiscus cannabinus Linn. in the treatment of urolithiasis and cholelithiasis

Q3 Medicine Digital Chinese Medicine Pub Date : 2023-06-01 DOI:10.1016/j.dcmed.2023.07.008

Swathi Suresh, Ankul Singh S, Chitra Vellapandian

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Abstract

Objective

To investigate the therapeutic effect of Hibiscus cannabinus Linn. (H. cannabinus) leaves on cholelithiasis and urolithiasis.

Methods

The study evaluated the effect of aqueous leaf extract of H. cannabinus on thiouracil and cholesterol cholic acid diet induced cholelithiasis in BALB/c mice and ethylene glycol induced urolithiasis in Wistar rats. Three doses of aqueous extract (40, 80, and 160 mg/kg) were selected to evaluate the effectiveness in cholelithiasis in mice; another three doses of aqueous extract (400, 800, and 1600 mg/kg) were administered for evaluating the effect on urolithiasis in rats. Biochemical parameters such as biliary cholesterol, biliary phospholipid, and bile acid were determined in cholelithiasis model. Similarly, 24-hour urine output, urinary parameters such as creatinine, uric acid, protein, urea, presence of calcium oxalate crystals, red blood cells (RBCs), and pyuria were determined in urolithiasis model.

Results

Statistically significant differences were noted in the biliary and urinary parameters after administrating three test doses of H. cannabinus aqueous extract (P < 0.05).

Conclusion

H. cannabinus was found to be effective against high fat lithogenic diet urolithiasis and cholelithiasis.

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大麻木槿的临床前评价。在治疗尿石症和胆石症

目的观察木槿的治疗作用。（大麻属）在胆结石和尿石症上的叶子。方法观察大麻素叶提取物对硫氧嘧啶和胆固醇-胆酸饮食诱导的BALB/c小鼠胆结石和乙二醇诱导的Wistar大鼠尿石的影响。选择三种剂量的水提取物（40、80和160 mg/kg）来评估对小鼠胆结石的有效性；另外三个剂量的水提取物（400、800和1600mg/kg）用于评估对大鼠尿石症的影响。在胆结石模型中测定胆汁胆固醇、胆汁磷脂和胆汁酸等生化参数。类似地，在尿石症模型中测定24小时尿量、尿参数，如肌酸酐、尿酸、蛋白质、尿素、草酸钙晶体的存在、红细胞（RBCs）和脓尿。结果给予三个试验剂量的大麻素水提取物后，胆汁和尿液参数差异有统计学意义（P<；0.05）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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