Collection and evaluation of semen collected from jaguarundi (Puma yagouaroundi) through urethral catheterization and electroejaculation

Dieferson da Costa Estrela , Bruna de Oliveira Mendes , Guilherme Malafaia , Antonio Campanha Martinez , Jessica da Silva Paulino , Cristina Harumi Adania , Nei Moreira
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导尿和电射精采集美洲豹精液的采集与评价
尿道导管插入术(Zambelli法)是一种通过电射精收集精液的替代技术。尽管在一些研究中,个体和少量样本之间存在相当大的差异,但对野生猫科动物的记录结果已在10种猫科动物中显示出其功效。鉴于导尿技术的创新潜力,本研究的目的是评估应用于jaguarundi(Puma yagouaroundi)的电射精后的精液采集方案,并将其与单独的电射精进行比较。对雄性(n=7)进行精液采集(n=5)。通过导尿(UC)采集精液,然后进行电射精(UC-EEJ,n=3),或者通过电射精(EEJ,n=2)采集精液。UC技术对57%的动物(n=4)的精液采集有效,但在所有采集中,没有一只动物能够提供精液。结果用平均值±SD表示。UC在18次采集试验中采集了6份精液样本,平均体积达到3.53±0.69µL,但只有一份样本显示出冷冻程序所需的质量(活力3、80%活力和88%活力)。其他样品活力为1.86±0.86,活力为21.32±12.59%,活力为27.6±13.05%。由于样本量小,无法评估其精液浓度。UC-EEJ和EEJ样本的活力分别为3±0.93和3.13±0.67;运动率分别为52.50±25.36%和54.09±20.47%;活力分别为58.50±24.50%和62.78±16.28%。这些样本比UC样本具有更好的定性参数,但它们之间没有差异。总之,UC技术在精液采集中是有效的,但通过它采集的样本质量不符合冷冻保存的标准;它们的值低于两种测试的电射精模式。
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来源期刊
Theriogenology wild
Theriogenology wild Animal Science and Zoology, Veterinary Science
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