TL1A induces apoptosis via DR3 in grass carp (Ctenopharyngodon idella)

IF 2.2 Q2 FISHERIES Fish and shellfish immunology reports Pub Date : 2023-03-15 DOI:10.1016/j.fsirep.2023.100090

Kangyong Chen , Qianzi Xiu , Qingyu Min , Xingxing Cheng , Hehe Xiao , Zhao Jia , Jianhua Feng , Yanjie Shi , Qianli Zhuo , Junya Wang , Jun Zou

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引用次数: 1

Abstract

Tumor necrosis factor like ligand 1A (TL1A), a member of TNF superfamily, regulates inflammatory response and immune defense. TL1A homologues have recently been discovered in fish, but their functions have not been studied. In this study, a TL1A homologue was identified in grass carp (Ctenopharyngodon idella) and its bioactivities were investigated. The grass carp tl1a (Citl1a) gene was constitutively expressed in tissues, with the highest expression detected in the liver. It was upregulated in response to infection with Aeromonas hydrophila. The recombinant CiTL1A was produced in bacteria and was shown to stimulate the expression of il1β, tnfα, caspase 8 and ifnγ in the primary head kidney leucocytes. In addition, co-immunoprecipitation assay revealed that CiTL1A interacted with DR3 and induced apoptosis via activation of DR3. The results demonstrate that TL1A regulates inflammation and apoptosis and is involved in the immune defense against bacterial infection in fish.

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TL1A通过DR3诱导草鱼细胞凋亡

肿瘤坏死因子样配体1A（TL1A）是TNF超家族的一员，调节炎症反应和免疫防御。TL1A同源物最近在鱼类中被发现，但其功能尚未得到研究。本研究在草鱼（Ctenopharyngodon idella）中鉴定了一种TL1A同源物，并对其生物活性进行了研究。草鱼tl1a（Citl1a）基因在组织中组成性表达，在肝脏中表达最高。它在感染嗜水气单胞菌时被上调。重组CiTL1A在细菌中产生，并显示出刺激原发性头肾白细胞中il1β、tnfα、caspase 8和ifnγ的表达。此外，共免疫沉淀分析显示，CiTL1A与DR3相互作用，并通过激活DR3诱导细胞凋亡。结果表明，TL1A调节鱼类的炎症和细胞凋亡，并参与对细菌感染的免疫防御。

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