Immunomodulatory Effects of Modified Bovine Colostrum, Whey, and Their Combination with Other Natural Products: Effects on Human Peripheral Blood Mononuclear Cells

IF 1.6 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Current Therapeutic Research-clinical and Experimental Pub Date : 2023-01-01 DOI:10.1016/j.curtheres.2023.100720

Xuesheng Han PhD, FACN , David Vollmer PhD , Elena Y. Enioutina MD, PhD

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Abstract

Background

Many natural products have immunomodulatory properties. However, the mechanism of immunomodulatory activities are poorly understood.

Objectives

This study evaluated the influence of bovine colostrum products, a whey product, or their combinations with other natural products on human peripheral blood mononuclear cells’ (PBMC) ability to produce cytokines upon activation.

Methods

PBMCs were pretreated with ultrafiltered colostrum, nano-filtered bovine colostrum, egg yolk extract, a botanical blend, colostrum + egg yolk extract, colostrum + egg yolk + botanical blend, and fermented whey and then stimulated with lipopolysaccharide or phytohemagglutinin. Cytokine production was measured by the Luminex assay.

Results

All study products demonstrated immunomodulatory properties by regulating cytokines production by activated PBMCs. Ultrafiltered colostrum alone displayed the highest immune stimulatory activity. It stimulated proinflammatory cytokine production by lipopolysaccharide-activated PBMCs and suppressed cytokine production by phytohemagglutinin-activated cells. Other study products mainly suppressed cytokine release by both cell types. The immunomodulatory properties depended upon the dose of the products used in the study.

Conclusions

All tested products modulated innate and adaptive immune cell activities. Most of the products demonstrated anti-inflammatory properties, except ultrafiltered colostrum, which stimulated the lipopolysaccharide-activated PBMC production of inflammatory cytokines. These products can be potentially used to support overall immune health.

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改良牛初乳、乳清及其与其他天然产物的联合免疫调节作用：对人外周血单个核细胞的影响

背景许多天然产物具有免疫调节特性。然而，人们对免疫调节活性的机制知之甚少。目的本研究评估了牛初乳产品、乳清产品或其与其他天然产品的组合对人外周血单核细胞（PBMC）激活后产生细胞因子的能力的影响。方法用超滤牛初乳、纳米过滤牛初乳、蛋黄提取物、植物混合物、初乳对PBMC进行预处理 + 蛋黄提取物、初乳 + 蛋黄 + 植物混合物和发酵乳清，然后用脂多糖或植物血凝素刺激。通过Luminex测定法测量细胞因子的产生。结果所有研究产品均通过调节活化PBMC产生的细胞因子而表现出免疫调节特性。单独的超滤初乳显示出最高的免疫刺激活性。它刺激脂多糖激活的PBMC产生促炎细胞因子，并抑制植物血凝素激活细胞产生细胞因子。其他研究产品主要抑制两种细胞类型的细胞因子释放。免疫调节特性取决于研究中使用的产品的剂量。结论所有测试产品都调节先天免疫细胞和适应性免疫细胞的活性。除超滤初乳外，大多数产品都表现出抗炎特性，超滤初乳刺激脂多糖激活的PBMC产生炎性细胞因子。这些产品有可能用于支持整体免疫健康。

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来源期刊

Current Therapeutic Research-clinical and Experimental 医学-药学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

3 months

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