Relative mutagenic effectiveness and efficiency of chemical mutagens (Caffeine and EMS) and heavy metals [(Pb(NO3)2 and Cd(NO3)2)] in developing chlorophyll and morphological mutants in lentil

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-08-01 DOI:10.1016/j.mrgentox.2023.503668

Durre Shahwar , Zeba Khan , Mohammad Yunus Khalil Ansari , Younghoon Park

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Abstract

Mutagenic effectiveness and efficiency are the utmost vital indices to determine the effective and efficient mutagenic concentrations for the generation of high frequency of desirable mutation in mutation breeding. Nevertheless, there are meager study are available for employing effective and efficient concentration of caffeine, EMS, Pb(NO₃)₂ and Cd(NO₃)₂ for the crop improvement. Thus, the current study was performed to assess the mutagenic effectiveness and efficiency of caffeine, EMS and heavy metals [Pb(NO₃)₂ Cd(NO₃)₂] and to determine the genetic variability of M₂ and M₃ lentil mutant lines. The frequency of different chlorophyll and chromophyll mutation was found highest at moderate and higher concentrations of chemical mutagens and heavy metals in M₁ and M₂ generation. The highest effectiveness was in 20 ppm Cd(NO₃)₂, followed by 20 ppm Pb(NO₃)₂, 0.10% EMS, and 0.10% caffeine. The present investigation also showed lower doses of caffeine, EMS, Pb(NO₃)₂, and Cd(NO₃)₂ were more efficient than higher concentrations, and caffeine was found more efficient followed by EMS, Pb(NO₃)₂, and Cd(NO₃)₂. Furthermore, a broad spectrum of viable mutations affecting different morphological characters of the plants viz., leaves, plant height, growth habits, flowers, pods, and seeds in M₂ and M₃ generation were recorded. Ten morphological mutants showing acceptable agronomic and horticultural features were identified, as genetic resources for further breeding.

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化学诱变剂（咖啡因和EMS）和重金属[（Pb（NO3）2和Cd（NO3

诱变有效性和效率是确定诱变育种中产生高频率期望突变的有效和高效诱变浓度的最重要指标。然而，关于采用有效和高效浓度的咖啡因、EMS、Pb（NO3）2和Cd（NO 3）2进行作物改良的研究却很少。因此，本研究旨在评估咖啡因、EMS和重金属[Pb（NO3）2Cd（NO3，2]的诱变效果和效率，并确定M2和M3扁豆突变系的遗传变异性。在M1和M2代中，不同叶绿素和叶绿素的突变频率在中等和较高浓度的化学诱变剂和重金属下最高。有效性最高的是20 ppm Cd（NO3）2，其次是20 ppm Pb（NO3，0.10%EMS和0.10%咖啡因。本研究还表明，低剂量的咖啡因、EMS、Pb（NO3）2和Cd（NO3。此外，在M2和M3代中，记录了影响植物不同形态特征的广泛可行突变，即叶片、株高、生长习性、花、荚和种子。鉴定出10个表现出可接受的农艺和园艺特征的形态突变体，作为进一步育种的遗传资源。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.