The histidine phosphatase LHPP of Penaeus vannamei is involved in shrimp hemocytes apoptosis

IF 2.2 Q2 FISHERIES Fish and shellfish immunology reports Pub Date : 2023-06-26 DOI:10.1016/j.fsirep.2023.100109

Zhongyan Wang , Yueling Zhang , Jude Juventus Aweya , Zhongyang Lin , Defu Yao , Zhihong Zheng

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引用次数: 1

Abstract

LHPP (Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase) is a protein histidine phosphatase that modulates a hidden posttranslational modification called histidine phosphorylation. LHPP also acts as a tumor suppressor, which plays a pivotal role in various cellular processes. However, whether LHPP participates in the regulation of invertebrate's immunity is still unknown. Here we characterized a LHPP homolog in P. vannamei (designated PvLHPP), with a 807 bp length of open reading frame (ORF) encoding a putative protein of 268 amino acids. Sequence analysis revealed that PvLHPP contains a typical hydrolase 6 and hydrolase-like domain, which was conserved from invertebrate to vertebrate. PvLHPP was ubiquitously expressed in tissues and induced in hemocyte and hepatopancreas by Vibrio parahaemolyticus, Streptococcus iniae and white spot syndrome virus (WSSV) challenge, indicating that PvLHPP participated in the immune responses. Moreover, silencing of PvLHPP followed by V. parahaemolyticus inhibited hemocyte apoptosis. This study enriches our current insight on shrimp immunity, and provides novel perspective to understand immune-regulatory role of PvLHPP.

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凡纳滨对虾组氨酸磷酸酶LHPP参与对虾血细胞凋亡

LHPP (Phospholysine phosphohitidine Inorganic焦磷酸盐磷酸酶)是一种蛋白质组氨酸磷酸酶，它调节一种被称为组氨酸磷酸化的隐藏翻译后修饰。LHPP还作为肿瘤抑制因子，在多种细胞过程中发挥关键作用。然而，LHPP是否参与无脊椎动物免疫的调节尚不清楚。本研究鉴定了南美扁豆中LHPP同源物(命名为PvLHPP)，其开放阅读框(ORF)长度为807 bp，编码268个氨基酸的推定蛋白。序列分析表明，PvLHPP含有一个典型的水解酶6和水解酶样结构域，该结构域在无脊椎动物和脊椎动物中都是保守的。PvLHPP在组织中普遍表达，并在白细胞和肝胰腺中被副溶血性弧菌、牛链球菌和白斑综合征病毒(WSSV)攻击诱导，表明PvLHPP参与了免疫应答。此外，沉默PvLHPP后再沉默副溶血性弧菌可抑制血细胞凋亡。本研究丰富了我们目前对对虾免疫的认识，为理解PvLHPP的免疫调节作用提供了新的视角。

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