{"title":"Molecular characterization of a begomovirus associated with leaf crumpling and severe mosaic disease of bell pepper","authors":"Priya Lager, Yogesh Kumar","doi":"10.1007/s13314-023-00517-y","DOIUrl":null,"url":null,"abstract":"<div><p>Bell pepper plant leaves showing typical begomovirus-like symptoms (crumpling, mosaic, and stunting) were collected from Jalandhar region of Punjab, India. Total DNA was isolated from symptomatic plant leaves, and species-specific primers were used to amplify and detect begomovirus. The full-length genome was amplified using the rolling circle amplification method, cloned into the pBluescript II KS (+) vector, and sequenced. A BLAST search of the current DNA-A isolate revealed the highest percentage of identity (98.83%) with other isolates. Betasatellite sequences on BLAST search showed a close similarity of 98.10% with other isolates. Phylogenetic analysis with other distinct begomovirus species revealed that the virus’s current DNA-A was closely related to the <i>Papaya leaf crumple virus</i> and the <i>Chenopodium leaf distortion virus</i>. For the present isolates of DNA-A and betasatellite, no major recombination event was found. In the present study, molecular identification and characterization of the <i>Papaya leaf crumple virus</i> with <i>Papaya leaf curl betasatellite</i> adds another host, and to the best of our knowledge, this is the first report of the present virus infecting the bell pepper plant worldwide.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australasian Plant Disease Notes","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13314-023-00517-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Bell pepper plant leaves showing typical begomovirus-like symptoms (crumpling, mosaic, and stunting) were collected from Jalandhar region of Punjab, India. Total DNA was isolated from symptomatic plant leaves, and species-specific primers were used to amplify and detect begomovirus. The full-length genome was amplified using the rolling circle amplification method, cloned into the pBluescript II KS (+) vector, and sequenced. A BLAST search of the current DNA-A isolate revealed the highest percentage of identity (98.83%) with other isolates. Betasatellite sequences on BLAST search showed a close similarity of 98.10% with other isolates. Phylogenetic analysis with other distinct begomovirus species revealed that the virus’s current DNA-A was closely related to the Papaya leaf crumple virus and the Chenopodium leaf distortion virus. For the present isolates of DNA-A and betasatellite, no major recombination event was found. In the present study, molecular identification and characterization of the Papaya leaf crumple virus with Papaya leaf curl betasatellite adds another host, and to the best of our knowledge, this is the first report of the present virus infecting the bell pepper plant worldwide.
从印度旁遮普省贾朗达尔地区收集的甜椒植物叶片显示典型的begomovirus样症状(皱褶、花叶和发育不良)。从有症状的植物叶片中分离总DNA,利用种特异性引物扩增和检测begomavirus。采用滚圈扩增法扩增全长基因组,克隆到pBluescript II KS(+)载体上,测序。目前的DNA-A分离物的BLAST搜索显示与其他分离物的同源性最高(98.83%)。BLAST检索的betasatsatellite序列与其他分离株的相似度为98.10%。与其他不同种begomavirus的系统发育分析表明,该病毒目前的DNA-A与木瓜叶片皱缩病毒和Chenopodium叶片扭曲病毒密切相关。对于目前分离的DNA-A和betasatellat,没有发现重大的重组事件。本研究利用木瓜卷曲betasatellat增加了另一个寄主,对木瓜皱叶病毒进行了分子鉴定和鉴定,据我们所知,这是目前世界上第一次报道该病毒侵染甜椒植物。
期刊介绍:
Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.