Increased IFN-γ+ and TNF-α+ mucosal-associated invariant T cells in patients with aplastic anemia

IF 2.3 3区医学 Q3 MEDICAL LABORATORY TECHNOLOGY Cytometry Part B: Clinical Cytometry Pub Date : 2023-02-13 DOI:10.1002/cyto.b.22115

Xiaohui Chen, Yuping Zhang, Yikai Zhang, Yue Zhang, Shunqing Wang, Zhi Yu, Xiaoen Liu, Guixuan Huang, Lixing Guo, Xueqin Li, Xianfeng Zha, Yangqiu Li, Bo Li

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引用次数: 2

Abstract

Background

Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal-associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA.

Methods

In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry.

Results

We found that the percentage of circulating MAIT cells was generally higher for CD3⁺, CD8⁺, and CD8⁻ T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL-18Rα-, NKG2D-, IFN-γ-, and TNF-α- positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN-γ⁺ CD3⁺ or TNF-α⁺CD8⁻ MAIT cells had a significant negative correlation with the absolute neutrophil count.

Conclusions

We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN-γ and TNF-α production and may contribute to the pathogenesis of AA.

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再生障碍性贫血患者IFN-γ+和TNF-α+粘膜相关不变T细胞增加

再生障碍性贫血（AA）是一种自身免疫性疾病，T细胞活化异常。据报道，非常规T细胞，即粘膜相关不变T细胞（MAIT），在几种自身免疫性疾病中发挥着重要作用，但尚不清楚它们是否与AA有关，流式细胞术检测AA中MAIT细胞的细胞因子特性。结果我们发现，与健康人相比，AA患者的CD3+、CD8+和CD8−T细胞的循环MAIT细胞百分比通常更高。此外，AA患者中IL-18Rα、NKG2D-、IFN-γ和TNF-α阳性MAIT细胞的百分比也显著较高。此外，IFN-γ+CD3+或TNF-α+CD8−MAIT细胞的百分比与中性粒细胞绝对计数呈显著负相关。结论我们首次在AA患者中观察到MAIT细胞。MAIT细胞与较高频率的IFN-γ和TNF-α产生有关，可能参与AA的发病机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytometry Part B: Clinical Cytometry 医学-病理学

CiteScore

6.80

自引率

32.40%

发文量

审稿时长

>12 weeks

期刊介绍： Cytometry Part B: Clinical Cytometry features original research reports, in-depth reviews and special issues that directly relate to and palpably impact clinical flow, mass and image-based cytometry. These may include clinical and translational investigations important in the diagnostic, prognostic and therapeutic management of patients. Thus, we welcome research papers from various disciplines related [but not limited to] hematopathologists, hematologists, immunologists and cell biologists with clinically relevant and innovative studies investigating individual-cell analytics and/or separations. In addition to the types of papers indicated above, we also welcome Letters to the Editor, describing case reports or important medical or technical topics relevant to our readership without the length and depth of a full original report.