Andrographis paniculata (Burm. f.) Nees extract ameliorates insulin resistance in the insulin-resistant HepG2 cells via GLUT2/IRS-1 pathway.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Archives of Physiology and Biochemistry Pub Date : 2024-12-01 Epub Date: 2023-10-25 DOI:10.1080/13813455.2023.2273221

Sri Ningsih, Siska Andrina Kusumastuti, Nuralih Nuralih, Adam Arditya Fajriawan, Devi Permatasari, Prasetyawan Yunianto, Donny Ramadhan, Mayriska Tri Wulandari, Nisrina Firdausi, Nurhadi Nurhadi, Reni Giarni, Kurnia Agustini, Agung Eru Wibowo, Idah Rosidah, Tiya Novlita Rengganis, Ngatinem Ngatinem, Agus Himawan Subiantoro, Agus Supriyono

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Abstract

Hyperglycaemia is one condition related to inflammation leading to insulin signalling impairment. This study was conducted to investigate the insulin sensitivity improvement of Sambiloto (Andrographis paniculata (Burm. f.)) Nees extract in insulin resistance-induced HepG2 (IR-HepG2) cells by stimulating insulin sensitivities and inhibiting inflammatory response. Sambiloto extract at 2 µg/mL revealed glucose uptake stimulation and up-regulating GLUT-2 and IRS-1 gene expression, and inhibited pro-inflammatory cytokine IL-6 gene expression in IR-HepG2 cells. Phytochemical analysis showed that the total phenolic level and andrografolide content of Sambiloto extract were 2.91 ± 0.04% and 1.95%, respectively. This result indicated that Sambiloto extract ameliorated insulin resistance in high glucose-induced IR-HepG2 cells via modulating the IRS-1/GLUT-2 pathway due to IL-6 inhibition. These findings suggested that Sambiloto extract had potency as an anti-inflammatory and insulin-resistance improvement in IR-HepG2 cells.

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穿心莲f。)Nees提取物通过GLUT2/IRS-1通路改善胰岛素抵抗HepG2细胞的胰岛素抵抗。

高血糖是一种与炎症相关的疾病，导致胰岛素信号受损。本研究旨在通过刺激胰岛素敏感性和抑制炎症反应来研究三倍子提取物对胰岛素抵抗诱导的HepG2（IR-HepG2）细胞的胰岛素敏感性的改善作用。桑比洛托提取物2 µg/mL在IR-HepG2细胞中显示葡萄糖摄取刺激和上调GLUT-2和IRS-1基因表达，并抑制促炎细胞因子IL-6基因表达。植物化学分析表明，桑比洛提取物的总酚含量和穿心莲内酯含量分别为2.91 ± 分别为0.04%和1.95%。这一结果表明，桑比洛提取物通过调节由于IL-6抑制而引起的IRS-1/GUT-2途径，改善了高糖诱导的IR-HepG2细胞中的胰岛素抵抗。这些发现表明桑比洛托提取物在IR-HepG2细胞中具有抗炎和胰岛素抵抗改善作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.