The Role of Nrf2-ARE Signaling Pathway and Tatarinow Sweetflag Extract to Regulate the Acute Phase of Pilocarpine-Induced Epilepsy in Juvenile Rats.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2024-10-01 Epub Date: 2023-10-25 DOI:10.1007/s12033-023-00911-y

Guanghui Jin, Zhuo Wang, Wei Zhou, Guyue Li

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Abstract

To analyze the role of Nrf2-ARE signaling pathway in the regulation of the acute phase of pilocarpine-induced epilepsy in juvenile rats by Tatarinow Sweetflag Extract (TSE). One hundred and twenty SPF-grade Wistar male rats were were divided into five groups by random number table method, namely, normal group, model group, low-dose TSE group, high-dose TSE group, low-dose TSE + Nrf2 inhibitor Brusatol group (low-dose TSE + BRU group), and high-dose TSE + Nrf2 inhibitor Brusatol group (high-dose TSE + BRU group), with 20 rats in each group. The success rate of modelling in the model group, low-dose TSE group, high-dose TSE group, low-dose TSE + BRU group, high-dose TSE + BRU group were 60.00% (12/20), 65.00% (13/20), 65.00% (13/20), 70.00% (14/20), and 70.00% (14/20), respectively, showing no significant difference (P > 0.05). The latency and incidence of class IV and V, discharge amplitude as well as frequency of rats in the low- and high-dose TSE groups were lower than those in the model group (P < 0.05); the lipid peroxide and malondialdehyde concentrations in hippocampal tissues in the low- and high-dose TSE groups were lower than those in the model group (P < 0.05); The Nrf2, NQO-1 and HO- 1 protein and mRNA expression levels were increased in the low- and high-dose TSE groups compared with the model group (P < 0.05). The therapeutic effect of TSE in rats with acute epilepsy was satisfactory, and its mechanism of action may be related to activation of Nrf2-ARE signaling pathway to reduce the degree of oxidative stress.

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Nrf2-ARE信号通路及烟叶提取物对匹罗卡品致痫大鼠急性期的调节作用

分析Nrf2-ARE信号通路在Tatarinow Sweetflag提取物（TSE）调节毛果芸香碱诱导的幼年大鼠癫痫急性期中的作用。采用随机数表法将120只SPF级Wistar雄性大鼠分为5组，即正常组、模型组、TSE低剂量组、TSE高剂量组、 + Nrf2抑制剂Brusatol组（低剂量TSE + BRU组）和高剂量TSE + Nrf2抑制剂Brusatol组（高剂量TSE + BRU组），每组20只。模型组、低剂量TSE组、高剂量TSE小组、低剂量TSE的建模成功率 + BRU组，高剂量TSE + BRU组分别为60.00%（12/20）、65.00%（13/20）、6.500%（13/20%）、70.00%（14/20）和70.00%（14/20），差异无统计学意义（P > 0.05）。TSE低、高剂量组大鼠的潜伏期、IV、V级发生率、放电幅度和频率均低于模型组（P

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.