Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks.

IF 2.1 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS Proteome Science Pub Date : 2023-10-24 DOI:10.1186/s12953-023-00222-9

Yaomei Wang, Yuchao Liu, Tingting Cao, Chunyuan Shi, Zili Ren, Yanling Zhao

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Abstract

Background: Proteins related to sperm motility and sperm morphology have an important impact on sperm function such as metabolism, motility and fertilisation etc. An understanding of the key proteins related to semen quality in Niangya yaks would help to provide support for breeding. However, the key proteins that affect semen quality in Niangya yaks remain unclear.

Methods: Herein, we applied tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC‒MS/MS) to analyze the expression levels of sperm proteins in groups of high- and low-quality semen from Niangya yaks. And fifteen differentially expressed proteins (DEPs) were randomly selected for expression level validation by parallel reaction monitoring (PRM).

Results: Of the 2,092 quantified proteins, 280 were identified as DEPs in the high-quality group versus the low-quality group. Gene Ontology (GO) analysis revealed that in terms of biological pathways, the DEPs were mainly involved in metabolic processes, cell transformation processes, and single organism metabolic processes. In terms of cell composition, the DEPs were mainly located in the cell membrane, organelle, molecular complex. In terms of molecular functions, the most abundant functions of the DEPs were catalytic activity, binding activity, transport activity, and enzyme regulation activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were mainly involved in the cytokine and cytokine receptor interaction, notch signaling pathway, lysine biosynthesis, renal function-related protein and proteasome pathway. From protein-protein interaction (PPI) analysis of DEPs involved in important pathways, 6 related proteins affecting the semen quality of Niangya yaks were identified. And the results of the PRM and TMT analysis were consistent.

Conclusions: The differential sperm proteomic analysis of high- and low-quality semen from Niangya yaks, revealed 6 proteins (PSMC5, PSMD8, PSMB3, HSP90AA1, UGP2 and HSPB1), were mainly concentrated in energy production and metabolism, might play important roles in semen quality, which could serve as candidates for the selection and breeding of Niangya yaks.

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定量蛋白质组学分析揭示了影响娘雅牦牛精液质量的关键蛋白。

背景：与精子活力和精子形态相关的蛋白质对精子的代谢、活力和受精等功能有重要影响。了解与娘惹牦牛精液质量相关的关键蛋白质将有助于为育种提供支持。然而，影响娘惹牦牛精液质量的关键蛋白质仍不清楚。方法：采用串联质谱（TMT）标记和液相色谱-串联质谱（LC-MS/MS）技术，分析了娘惹牦牛高、低质量精液中精子蛋白的表达水平。结果：在2092个定量蛋白质中，280个在高质量组和低质量组中被鉴定为DEP。基因本体论（GO）分析表明，就生物学途径而言，DEPs主要参与代谢过程、细胞转化过程和单体代谢过程。就细胞组成而言，DEP主要分布在细胞膜、细胞器、分子复合体中。就分子功能而言，DEPs最丰富的功能是催化活性、结合活性、转运活性和酶调节活性。京都基因与基因组百科全书（KEGG）分析显示，DEPs主要参与细胞因子和细胞因子受体的相互作用、notch信号通路、赖氨酸生物合成、肾功能相关蛋白和蛋白酶体通路。通过对参与重要途径的DEPs的蛋白质-蛋白质相互作用（PPI）分析，鉴定出6种影响娘惹牦牛精液质量的相关蛋白质。PRM和TMT分析的结果是一致的。结论：对年亚牦牛高、劣质精液进行精子蛋白质组学差异分析，发现6种蛋白质（PSMC5、PSMD8、PSMB3、HSP90AA1、UGP2和HSPB1）主要集中在能量生产和代谢过程中，可能对精液质量起重要作用，可作为年亚牦牛选育的候选蛋白。

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来源期刊

Proteome Science 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： Proteome Science is an open access journal publishing research in the area of systems studies. Proteome Science considers manuscripts based on all aspects of functional and structural proteomics, genomics, metabolomics, systems analysis and metabiome analysis. It encourages the submissions of studies that use large-scale or systems analysis of biomolecules in a cellular, organismal and/or environmental context. Studies that describe novel biological or clinical insights as well as methods-focused studies that describe novel methods for the large-scale study of any and all biomolecules in cells and tissues, such as mass spectrometry, protein and nucleic acid microarrays, genomics, next-generation sequencing and computational algorithms and methods are all within the scope of Proteome Science, as are electron topography, structural methods, proteogenomics, chemical proteomics, stem cell proteomics, organelle proteomics, plant and microbial proteomics. In spite of its name, Proteome Science considers all aspects of large-scale and systems studies because ultimately any mechanism that results in genomic and metabolomic changes will affect or be affected by the proteome. To reflect this intrinsic relationship of biological systems, Proteome Science will consider all such articles.