Thermo-alkali stable bacterial xylanase for deinking of copier paper.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-10-25 DOI:10.1186/s43141-023-00563-0
Girisha Malhotra, Shilpa S Chapadgaonkar
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Abstract

Background: The bleach-boosting capability of xylanases is well-known. The use of xylanase pre-treatment before the application of chemical bleach has multiple advantages including (i) lesser use of polluting chemicals of the traditional bleaching process; (ii) less damage to the cellulosic fibers, therefore better recyclability; and (iii) better brightness of chemical bleach. The major impediment in the application is the availability of commercial enzymes that are active at the elevated temperature and pH that exist during the industrial pulping process. In the present paper, xylanase having suitability for application in deinking is reported.

Results: The xylanase used showed high deinking potential. Optimal deinking was obtained at the xylanase dosing of 20U/g of the dried pulp at 60℃ for a treatment time of 1h. It could bring about a 50% reduction in the usage of chemical bleach that was applied after xylanase pre-treatment. The comparison of FTIR spectra showed changes in intensity without significant changes in the functional group signatures implying that there is negligible damage to the fiber strength in the xylanase pre-treatment process as compared to the chemical bleach process.

Conclusion: The xylanase used in this study was effective in deinking paper pulp and can be used for bio-bleaching of recycled paper.

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热碱稳定型细菌木聚糖酶用于复印纸脱墨。
背景:木聚糖酶的漂白增强能力是众所周知的。在应用化学漂白剂之前使用木聚糖酶预处理具有多种优点,包括(i)较少使用传统漂白工艺的污染化学品;(ii)对纤维素纤维的损伤较小,因此具有更好的可回收性;和(iii)化学漂白剂的亮度更好。应用中的主要障碍是在工业制浆过程中存在的在升高的温度和pH下具有活性的商业酶的可用性。本文报道了适用于脱墨的木聚糖酶。结果:木聚糖酶具有较高的脱墨潜力。当木聚糖酶用量为20U/g干浆,温度为60℃,处理时间为1h时,可获得最佳脱墨效果。它可以使木聚糖酶预处理后使用的化学漂白剂的使用量减少50%。FTIR光谱的比较显示强度的变化而官能团特征没有显著变化,这意味着与化学漂白工艺相比,木聚糖酶预处理工艺对纤维强度的损害可以忽略不计。结论:本研究所用木聚糖酶对废纸脱墨效果良好,可用于再生纸的生物漂白。
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