Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-07-01

K A Sofi, B Qureshi

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Abstract

Background: Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone.

Objective: To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of.

Materials and methods: SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN.

Results: SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS.

Conclusion: SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.

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条状吸管：用于卵母细胞玻璃化冷冻的成本效益高的替代冷冻装置。

背景：通过使用合适的冷冻装置提高冷却和升温速率，可以使用较低的冷冻保护剂浓度，并减少快速通过“危险”温度区造成的冷冻伤害。目的：评价新型条状吸管（SPS）对未成熟卵母细胞玻璃化后温育质量、活力和体外成熟的有效性。材料和方法：利用传统的法国迷你吸管，结合OPS和Cryotop系统的优点，制备SPS。未成熟绵羊卵母细胞在15%EG+15%DMSO中处理，装载在SPS上并浸入液氮（LN）中。结果：SPS解冻后形态存活率为90.9%，形态缺陷率为9.0%，存活率为96.4%，体外成熟率为51%。与OPS相比，SPS具有更高的解冻后存活率（86.5%vs 67.9%）和成熟率（57.7%vs 50.5%），形态缺陷较低（13.5%vs 32.1%）。在SPS（40.9%）和OPS（44.1%）的暖后卵母细胞形态异常中，Cumulus细胞损失最高，与传统的OPS相比，SPS可以作为一种成本效益高的卵母细胞玻璃化替代装置。Doi:10.54680/fr23410110212。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.